稻瘟病是水稻的主要病害，培育抗稻瘟病品种是防治稻瘟病的有效途径。本研究通过分子标记辅助选择与杂交育种相结合的方式，将稻瘟病抗性基因Pi1、Pi2和Pi9导入到三系杂交稻恢复系R225。对BC3F3代材料进行苗期和成熟期稻瘟病抗性鉴定，携带1个或2个抗性基因的目标株系抗性达到中抗以上水平，稻瘟病抗性显著高于各自的轮回亲本。SSR标记分析表明，改良株系的遗传背景回复率达到86.1%~95.3%。通过标记辅助选择获得的改良材料为三系杂交稻恢复系的培育提供了稻瘟病抗性亲本。
Rice blast is one of the most devastating diseases in rice. Cultivating rice blast resistant varieties is an effective way to control rice blast. Using marker assisted selection and backcrossing，blast resistance genes Pi1, Pi2 and Pi9 were introgressed into restorer parental lines R225. The BC3F3 progenies harboring Pi1, Pi2 or Pi9 were selected by linked markers. The lines carrying one or two target genes are significantly higher than their respective recurrent parent. Background SSR analysis showed that the recovery rate of the target lines reached 86.1%~95.3%. The improved lines can be used as candidate parental lines for rice breeding with blast resistance.
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