超级早稻中早39在多年的浙江省联品和区试中持久高抗稻瘟病，为明确其抗性遗传基础，用12个稻瘟病抗性基因Pi-d2、Pi-d3、Pi36、Pi37、Pi5、Pib、Piz、Pik-h、Piz-t、Pi9、Pi-ta 和Pi64的分子标记，对中早39进行抗性基因检测。结果表明，12对分子标记在中早39均有目的扩增条带；因Piz、Piz-t和 Pi9为复等位基因，中早39中至少含有10个稻瘟病抗性基因。这一结果为中早39的稻瘟病抗性遗传利用奠定了基础。
The super rice variety Zhongzao 39 showed high and durable resistant to rice blast.Twelve pairs of molecular markers for the target resistant genes: Pi-d2, Pi-d3, Pi36, Pi37, Pi5, Pib, Piz, Pik-h, Piz-t, Pi9, Pi-ta and Pi64, were used to understand the resistant genetic mechanism of Zhongzao 39. The results indicated that all of the molecular markers yielded positive bands with expected size. As Piz, Piz-t and Pi9 were allelic to each other, there were ten rice blast resistant genes were identified in Zhongzao 39. All these results provided valuable information to the utilization of Zhongzao 39 in rice blast resistant breeding.
[1] 夏令治，季芝娟，曾宇翔，等. 超级稻品种中早39的稻瘟病抗谱测定[J]. 中国稻米，2015，21（3）：69-72.
[2] 夏令治. 超级早稻中早39的抗稻瘟病遗传机理研究[D]. 北京：中国农业科学院，2015.
[3] 孙庚. 水稻抗瘟基因与稻瘟菌无毒基因的分布及稻瘟菌分子检测[D]. 长春：吉林大学，2012.
[4] Hayashi K, Yoshida H, Ashikawa I. Development of PCR-based allele specific and InDel marker sets for nine rice blast resistance genes [J]. Theor Appl Genet, 2006, 113: 251-260.
[5] Sharma T R, Madhav M S, Singh B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea [J]. Mol Genet Genomics, 2005, 274: 569-578.
[6] Tian D G, Chen Z J, Chen Z Q, et al. Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars[J]. Rice, 2016, 9（1）: 19.
[7] Jia Y L, Wang Z H, Singh P. Development of dominant rice blast Pi-ta resistance gene markers[J]. Crop Sci, 2002, 42: 2 145-2 149.
[8] Ma J, Lei C L, Xu X T, et al. Pi-64, encoding a novel CC-NBS-LRR protein, confers resistance to leaf and neck blast in rice [J]. Mol Plant-Microbe Interac, 2015, 28（4）: 558-568.
[9] Warude D, Preeti C, Joshi K, et al. DNA isolation from fresh and dry plant samples with highly acidic tissue extracts [J]. Plant Mol Biol Rep, 2003, 21: 467.
[10] Liang Y, Yan B Y, Peng Y L, et al. Molecular screening for identification of resistant genes in a germplasm （Oryza sativa L.） collection resistant to Magnaporthe oryzae [J]. Rice Sci, 2017, 24（1）: 41-47.
[11] 张羽，冯志峰，张先平，等. 5个稻瘟病抗性基因的基因型分型与抗性评价研究[J]. 西南农业学报，2014，27（5）：1 928-1 938.
[12] 阎勇，马增凤，秦钢，等. 华南常用籼稻亲本稻瘟病抗性评价及抗性基因鉴定[J]. 南方农业学报，2017，48（4）：587-593.
[13] 潘争艳，邱福林，吕桂兰，等. 辽星系列水稻品种稻瘟病抗性基因鉴定与抗性分析[J]. 江苏农业科学，2017，45（7）：87-90.
