控制水稻中胚轴伸长的QTL定位
QTLs Mapping for Mesocotyl Length in Rice
水稻中胚轴能够为幼苗破土提供动力，培育长中胚轴水稻品种有助于水稻直播技术的推广，因此，研究水稻中胚轴伸长具有重要的理论和现实意义。为分析调控水稻中胚轴伸长的遗传基础，以Asominori和IR24重组自交系（RIL）群体为材料，结合其连锁图谱，对2017年杭州收获种子的中胚轴长度性状进行QTL定位。结果表明，在Asominori/IR24重组自交系群体中共检测到3个控制中胚轴伸长的QTL位点，分别位于第2、第3和第7条染色体上，LOD值在2.34~3.41之间，单个QTL对表型贡献率在7.25%~11.07%之间。同时用Asominori遗传背景的IR24染色体片段代换系进行验证，qML2对应的代换家系CSSL12的中胚轴与Asominori相比显著伸长，qML7对应的代换家系CSSL37的中胚轴与Asominori相比显著缩短，从而验证了qML2和qML7位点的存在。与先前研究比较，qML3和qML7在不同群体不同环境下稳定表达。同时利用重组自交系群体对2018年杭州大田环境株高性状进行QTL定位，共检测到2个QTL位点，均与中胚轴QTL位点不重合，说明控制中胚轴伸长与控制株高有着不同的遗传基础。
牛世朋,吕育松,邬亚文,魏祥进,圣忠华,焦桂爱,胡时开,唐绍清* . 控制水稻中胚轴伸长的QTL定位[J]. 中国稻米, 2019 , 25(6) : 55 -59 . DOI: 10.3969/j.issn.1006-8082.2019.06.014
The Rice mesocotyl can provide the power for seedling soilbreaking. The rice varieties with long mesocotyl could promote the popularization of rice direct seeding technology. Therefore, it is of great theoretical and practical significance to study rice mesocotyl elongation. To analyze the genetic basis of rice mesocotyl elongation, the Asominori and IR24 recombinant inbred lines (RIL) and their linkage maps were used for the experiment. The seeds gained in 2017 in Hangzhou were cultivated and measured the length of the mesocotyl for the QTLs mapping for mesocotyl length. Three QTLs were detected which were located on chromosomes 2, 3 and 7 respectively, and the LOD ranged from 2.34 to 3.41. The contribution rate of QTL to phenotype is between 7.25% and 11.07%. At the same time, the result was tested by a series of single segment substitution lines. The CSSL12 corresponding to qML2 was significantly elongated compared with Asominori, and the CSSL37 mesocotyl corresponding to qML7 significantly shortened, which proved the result of the QTL qML2 and qML7 to be right. At the same time, QTL mapping of plant height trait was carried out and two QTLs were detected, which were not coincident with QTLs for the length of mesocotyl, which indicating that controlling mesocotyl elongation and controlling plant height have different genetic basis.
Key words： rice; mesocotyl; QTL; soil breaking ability; direct seeding
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