专论与研究

以种子电导率为指标定位水稻耐储藏QTL

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  • 浙江农林大学农业与食品科学学院,杭州 311300

网络出版日期: 2020-01-20

基金资助

浙江省农业(粮食)新品种选育重大科技专项(2016C02050-2)

Mapping of QTL for Rice Storability Based on Seed Electric Conductivity

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Online published: 2020-01-20

摘要

水稻长时间储藏后,种子活力下降,稻米品质劣变,往往造成重大损失。开展水稻耐储藏研究,遗传改良水稻的耐储藏特性越来越受到重视。本研究以秀水134为背景亲本,以扬稻6号为供体亲本构建的BC3F2高代回交群体为材料,以电导率为指标进行表型-分子标记基因型的单因素方差分析,来定位耐储藏QTL。以-log10(P)>2为阈值,共检测到16个耐储藏相关的QTL。其中,RM5455(相对加性效应为-12.93%)、RM1132(相对加性效应-13.76%)和RM248(相对加性效应-14.66%)位点区域均存在着效应较大的耐储藏QTL。

本文引用格式

曹玉洁#,费月新#,赵文佳,侯璐燕,王越,吴敏,许珊,吴洪恺* . 以种子电导率为指标定位水稻耐储藏QTL[J]. 中国稻米, 2020 , 26(1) : 46 -49 . DOI: 10.3969/j.issn.1006-8082.2020.01.010

Abstract

 Prolonged storage of rice tends to cause a lot of losses due to deterioration in seed vigor and grain quality. More and more attention is paid to genetic improvement of rice storability through carrying out genetic research on storability. In this study, an advanced backcross population was developed with Xiushui 134 as the background parent and Yangdao 6 as the donor parent. The one-way analysis of variance based on phenotype-molecular genotype was applied to map the QTLs for storability. A total of 16 QTLs associated with storability were detected at threshold value of -log10(P)>2. Among them, three major QTL were detected on the locus of RM5455 (the relative effect is -12.93%), RM1132 (the relative effect is -13.76%) and RM248 (the relative effect of -14.66%).

参考文献

[1]    LIN Q, JIANG Y, SUN A L, et al. Fine mapping of qSS-9, a major and stable quantitative trait locus, for seed storability in rice (Oryza sativa L.)[J]. Plant Breed, 2015, 134(3): 293-299.
[2]    SASAKI K, TAKEUCHI Y, MIURA K, et al. Fine mapping of a major quantitative trait locus, qLG-9, that controls seed longevity in rice (Oryza sativa L.) [J]. Theor Appl Genet, 2015, 128(4): 769-778.
[3]    KHALIQ A, ASLAM F, MATLOOB A, et al. Seed priming with selenium: consequences for emergence, seedling growth, and biochemical attributes of rice[J]. Biol Trace Elem Res, 2015, 166: 236-244.
[4]    ISTA (International Seed Testing Association). International rules for seed testing[M]. International Seed Testing Association, Bassersdorf, Switzerland, 2010.
[5]    周显青,张玉荣. 储藏稻谷品质指标的变化及其差异性[J]. 农业工程学报,2008,24(12):238-242.
[6]    SUSAN R M, LEONID T, YUNBI XU, et al, Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)[J]. DNA Res. 2002, 9(6): 199-207.
[7]    陈信波,罗泽民. 杂交水稻种子在贮存过程中生活力及生理生化变化的研究[J]. 杂交水稻,1994,9(3):32-34.
[8]    王振宝,王树春,徐国良. 一种种子活力的测定方法:电导率法[J]. 种子,1998(3):70.
[9]    高用明,朱军. 植物QTL定位方法的研究进展[J]. 遗传,2000,22(3):175-179.
[10]  阮成江,何祯祥,钦佩. 我国农作物QTL定位研究的现状和进展[J]. 植物学通报,2003,20(1):10-22.
[11]  杨德卫. 以9311为背景的日本晴染色体片段置换系的构建及相关QTL分析[D]. 南京:南京农业大学,2009.

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