以早籼稻浙辐802及其近等基因系fgl为材料，采用盆栽试验，于籽粒灌浆期在人工气候箱进行高温处理10 d（40℃/30℃，昼夜），研究高温下喷施蔗糖溶液对水稻结实率、千粒重和干物质积累及分配的影响。结果表明，籽粒灌浆期高温胁迫对结实率的影响较小，但千粒重显著下降，其中，浙辐802下降的幅度显著高于fgl。蔗糖在一定程度上可减轻高温胁迫对水稻粒重及同化物转运的抑制作用。高温下，蔗糖处理的千粒重显著高于对照，籽粒面积、周长及长度均高于对照。高温下，蔗糖对水稻植株同化物积累影响不大，但显著提高同化物在穗部的比例，即高温下蔗糖处理穗部干物质积累比例显著高于对照。鉴于此，笔者认为，蔗糖减轻籽粒灌浆期高温胁迫抑制的作用，主要在于影响同化物转运而不在于干物质积累。
A indica rice Zhefu 802 and its near-isogenic line fgl were subjected to high temperature conditions at 40℃/30℃ （day/night） for 10 d at grain filling stage. The rice plants was sprayed with 2.5% sucrose before heat stress imposed. The results indicated that heat stress at grain filling stage had less effect on seed setting rate, but reduced the kernel weight in both rice varieties significantly, which accompanied with lower ratio between panicle dry weight and total dry weight, rather than the dry matter weight accumulation. Rice plants treated with sucrose attained significantly higher kernel weight than that of H2O treatment under heat stress in both rice varieties. Similarly, the ratio between the panicle dry matter and total plant dry matter of the sucrose treatment was significantly higher than those of plants treated with H2O under heat stress. This suggested that sucrose could alleviate the damages on the kernel weight and assimilate distribution by heat stress at grain filling stage.
[1] 曹云英，段骅，杨立年，等. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因[J]. 作物学报，2008，34（12）： 2 134-2 142.
[2] 魏金连，潘晓华，邓强辉. 不同生育阶段夜温升高对双季水稻产量的影响[J]. 应用生态学报，2010，21（2）：331-337.
[3] 张桂莲，陈立云，张顺堂，等. 高温胁迫对水稻花粉粒性状及花药显微结构的影响[J]. 生态学报，2008（3）：1 089-1 097.
[4] JAGADISH S, CRAUFURD P, WHEELER T. High temperature stress and spikelet fertility in rice（Oryza sativa L. ）[J]. J Exp Bot, 2007, 58（7）: 1 627-1 635.
[5] PRASAD P V V, BOOTE K J, ALLEN L H, et al. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress [J]. Field Crop Res, 2006, 95: 398-411.
[6] ZHANG C X, LI G Y, CHEN T T, et al. Heat stress induces spikelet sterility in rice at anthesis through inhibition of pollen tube elongation interfering with auxin homeostasis in pollinated pistils [J]. Rice, 2018, 11（1）:14.
[7] 森谷国男，徐正进.水稻高温胁迫抗性遗传育种研究概况[J]. 杂交水稻，1992（1）：47-48.
[8] 刘博，韩勇，李建国，等. 灌浆期高温对水稻产量的影响[J]. 农产品加工（创新版），2011（4）：63-65.
[9] 符冠富，张彩霞，杨雪芹，等. 水杨酸减轻高温抑制水稻颖花分化的作用机理研究[J]. 中国水稻科学，2015，29（6）：637-647.
[10] ZHANG C X, FENG B H, CHEN T T, et al. Sugars, antioxidant enzymes and IAA mediate salicylic acid to prevent rice spikelet degeneration caused by heat stress [J]. Plant Growth Regul, 2017: 1-11.
[11] 王强，陈雷，张晓丽，等. 化学调控对水稻高温热害的缓解作用研究[J]. 中国稻米，2015，21（4）：80-82.
[12] ISLAM M R, FENG B H, CHEN T T, et al. Abscisic acid prevents pollen abortion under high temperature stress by mediating sugar metabolism in rice spikelets [J]. Physiologia Plantarum, 2018, 165（3）: 644-663.
[13] 尤翠翠. 水稻穗期高温热害的生理与分子机制[D]. 合肥：安徽农业大学，2013.
[14] 杨卫兵，王振林，尹燕枰，等. 外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响[J]. 中国农业科学，2011，44（13）：2 673-2 682.
[15] CHEN T T, ZHAO X, ZHANG C X, et al. Application of salicylic acid improves filling of inferior grains of rice during late maturity under mild cold stress[J]. Crop Sci, 2017, 57: 2 130-2 142.
[16] BONFIG K B, GABLER A, SIMON U K, et al. Post-translational derepression of invertase activity in source leaves via down-regulation of invertase inhibitor expression is part of the plant defense response [J]. Mol Plant. 2010, 3（6）: 1 037-1 048.
[17] RUAN Y L, JIN Y, YANG Y J, et al. Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat[J]. Mol Plant, 2010, 3（6）: 942-955.
[18] RUAN Y L. Signaling role of sucrose metabolism in development [J]. Mol Plant, 2012, 5（4）: 763-765.
[19] FANG C, HU X, SUN C, et al. Simultaneous determination of multi rice quality parameters using image analysis method [J]. Food Anal Methods, 2015, 8（1）: 70-78.
[20] JAGADISH S V K, MUTHURAJAN R, RANG Z W, et al. Spikelet proteomic response to combined water deficit and heat stress in rice （Oryza sativa, cv. N22） [J]. Rice, 2011, 4（1）: 1-11.
[21] LIN C J , LI C Y , LIN S K , et al. Influence of high temperature during grain filling on the accumulation of storage proteins and grain quality in rice （Oryza sativa L.） [J]. J Agr Food Chem, 2010, 58（19）: 10 545-10 552.
[22] 黄英金，罗永锋，黄兴作，等. 水稻灌浆期耐热性的品种间差异及其与剑叶光合特性和内源多胺的关系[J]. 中国水稻科学，1999，13（4）：205-210.
[23] 赵海燕，姚凤梅，张勇，等.长江中下游水稻开花灌浆期气象要素与结实率和粒重的相关性分析[J]. 中国农业科学，2006，41（9）： 1 765-1 771.
[24] 李木英，石庆华，胡志红，等. 高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响[J]. 中国农业科学，2007，40（8）：1 622 - 1 629.
[25] 程方民，蒋德安，吴平，等. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征[J].作物学报，2001，27（2）：201-206.
[26] 张国发，王绍华，尤娟，等. 结实期不同时段高温对稻米品质的影响[J]. 作物学报，2006，32（2）：283-287.
[27] 滕中华，智丽，宗学凤，等. 高温胁迫对水稻灌浆结实期叶绿素荧光、抗活性氧活力和稻米品质的影响[J]. 作物学报，2008，34（9）：1 662-1 666.
[28] 蔡水文，陈良碧. 水稻灌浆期温度对籽粒淀粉积累和FBP酶活性的影响[J]. 农业现代化研究，2004，25（4）：310-312.
[29] 小葉田亨，植向直哉，稲村達也，等. 子実への同化産物供給不足による高温下の乳白米発生[J]. 日本作物学会紀事，2004，73（3）：315-322.
[30] 王丰，程方民，刘奕，等. 不同温度下灌浆期水稻籽粒内源激素含量的动态变化[J]. 作物学报，2006，32（1）：25-29.
[31] COCK J H, YOSHIDA S. Accumulation of C14-labelled carbohydrate before fowering and its subsequent redistribution and respiration in the rice plant [J]. Proc Crop Sci Soc Jpn, 1972, 41（2）: 226-234.
[32] WOBUS U, WEBER H. Sugars as signal molecules in plant seed development[J]. Biol Chem, 1999, 380: 937-944.
[33] O’HARA L E, PAUL M J, WINGLER A. How do sugars regulate plant growth and development? New insight into the role of trehalose-6-phosphate [J]. Mol Plant, 2013, 6（2）: 261-74.
[34] CHEN T T, LI G, ISLAM M R, et al. Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship [J]. BMC Plant Biol, 2019, 19（1）:525.
[35] ZHANG C X, FENG B H, CHEN T T, et al. Heat stress-reduced kernel weight in rice at anthesis is associated with impaired source-sink relationship and sugars allocation [J]. Environ Exp Bot, 2018, 155: 718-733.
[36] WANG Y L, ZHANG Y K, SHI Q H, et al. Decrement of sugar consumption in rice young panicle under high temperature aggravates spikelet number reduction [J]. Rice Sci, 2020, 27（1）: 44-55.
