通过室内恒温培养试验,对酸化水稻土单独添加硫酸铵以及同时添加硫酸铵和葡萄糖后发现,添加硫酸铵后短时间内(2 d)就引起pH值下降和铵态氮转化为硝态氮,外源添加葡萄糖后进一步引起pH值下降和促进铵态氮向硝态氮转化,这种差异在长期(120 d)培养后更加显著。硫酸铵显著引起NO和N2O气体释放,外源添加葡萄糖后进一步促进两种气体的释放。测定土壤微生物丰度发现,两种温室气体的释放与土壤中氨氧化细菌、氨氧化古菌和反硝化细菌存在显著相关性。在短期培养时,氨氧化细菌和反硝化细菌丰度的差异导致了同时添加葡萄糖处理的温室气体释放量高于单纯的添加硫酸铵培养组;在长期培养时,则是氨氧化细菌、氨氧化古菌和反硝化细菌导致了这种差异。
A acidified paddy soil was used to incubated with ammonium sulfate along or ammonium sulfate together with glucose, the results showed that addition of ammonium sulfate significantly accelerated the soil acidification, increased the transformation of NH4+ to NO3- at 2 d of incubation, and these differences was more significant after a long-term (120 days) incubation. Ammonium sulfate significantly caused the release of NO and N2O, and exogenous glucose further promoted the release of the two greenhouse gases. There was a significant correlation among the release of two greenhouse gases and ammonia oxidizing bacteria, ammonia oxidizing archaea and denitrifying bacteria. In the short-term incubation (2 days), the different abundance of ammonia oxidizing bacteria and denitrifying bacteria led to the higher greenhouse gas emission from the simultaneous addition of ammonium sulfate and glucose treatments than the simple ammonium sulfate treatment; in the long-term incubation(120 days), ammonia oxidizing bacteria, ammonia oxidizing archaea and denitrifying bacteria led to this difference.
[1] KOCHIAN L V, PINEROS M A, HOEKENGA O A. The physiology, genetics and molecular biology of plant aluminum resistance and toxicity, in root physiology: from gene to function [J]. Plant and Soil, 2005, 274: 175-195.
[2] 赵其国. 中国东部红壤地区土壤退化的时空变化, 机理及调控[M]. 北京:科学出版社,2002.
[3] 张桃林. 中国红壤退化机制与防治 [M]. 北京:中国农业出版版社,1999.
[4] VENTEREA R T, GROFFMAN P M, VERCHOT L V, et al. Nitrogen oxide gas emissions from temperate forest soils receiving long-term nitrogen inputs [J]. Global Change Biology, 2003, 9(3): 346-357.
[5] GROFFMAN, P M, GOLD A J, ADDY K. Nitrous oxide production in riparian zones and its importance to national emission inventories [J]. Chemosphere-Global Change Science, 2000, 2(3): 291-299.
[6] GALLOWAY J N, ABER J D, ERISMAN J W, et al. The nitrogen cascade [J]. Bioscience, 2003, 53(4): 341-356.
[7] WILLIAMS E, G. HUTCHINSON, FEHSENFELD F. NOx and N2O emissions from soil [J]. Global Biogeochemical Cycles, 1992, 6(4): 351-388.
[8] WRAGE N, VELTHOF G L, BEUSICHEM M L V, et al. Role of nitrifier denitrification in the production of nitrous oxide [J]. Soil biology and Biochemistry, 2001, 33(12): 1 723-17 32.
[9] MENG L, DING W X, CAI Z C. Long-term application of organic manure and mineral fertilizer on N2O and CO2 emissions in a red soil from cultivated maize-wheat rotation in China [J]. Agricultural Sciences in China, 2011, 10(11): 1 748-1 757.
[10] 佟德利, 徐仁扣. 三种氮肥对红壤硝化作用及酸化过程影响的研究[J]. 植物营养与肥料学报,2012,18(4):853-859.
[11] YONG X, CUI Y, CHEN L, et al. Dynamics of bacterial communities during solid-state fermentation using agro-industrial wastes to produce poly-γ-glutamic acid, revealed by real-time PCR and denaturing gradient gel electrophoresis (DGGE) [J]. Applied Microbiology and Biotechnology, 2011, 92(4): 717-725.
[12] WENG B, XIE X, YANG J, et al. Research on the nitrogen cycle in rhizosphere of Kandelia obovata under ammonium and nitrate addition [J]. Marine Pollution Bulletin, 2013, 76(1-2): 227-40.
[13] BRAKER G A, FESEFELDT, WITZEL K P. Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples [J]. Applied and Environmental Microbiology, 1998, 64(10): 3 769 - 3 775.
[14] THROBACK I N, ENWALL K, JARVIS A, et al. Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE [J]. FEMS Microbiology Ecology, 2004, 49(3): 401-417.
[15] SCHROEDER K, OKUBARA P A, TAMBONG J T, et al. Identification and quantification of pathogenic Pythium spp. from soils in eastern Washington using real-time polymerase chain reaction [J]. Phytopathology, 2006, 96(6): 637-647.
[16] SCHROEDER K, OKUBARA P A, TAMBONG J T, et al. Regulation of nitric oxide emissions from forest and rangeland soils of western North America [J]. Ecology, 2002, 83(8): 2 278-2 292.
[17] CHEN J, WU F H, XIAO Q, et al. Diurnal variation of nitric oxide emission flux from a mangrove wetland in Zhangjiang River Estuary, China [J]. Estuarine, Coastal and Shelf Science, 2010, 90(4): 212-220.
[18] SIMEK M, COOPER J. The influence of soil pH on denitrification: progress towards the understanding of this interaction over the last 50 years [J]. European Journal of Soil Science, 2002, 53(3): 345-354.
[19] JIANG X, HOU X, ZHOU X, et al. pH regulates key players of nitrification in paddy soils [J]. Soil Biology and Biochemistry, 2015, 81 (1): 9-16.
[20] ZHENG X, HUANG Y, WANG Y, et al. Seasonal characteristics of nitric oxide emission from a typical Chinese rice–wheat rotation during the non-waterlogged period [J]. Global Change Biology, 2003, 9(2): 219-227.
[21] WANG X, BI X H, SHENG G Y, et al. Chemical composition and sources of PM10 and PM2. 5 aerosols in Guangzhou, China [J]. Environmental Monitoring and Assessment, 2006, 119(1-3): 425-439.
[22] GODDE M, CONRAD R. Simultaneous measurement of nitric oxide production and consumption in soil using a simple static incubation system, and the effect of soil water content on the contribution of nitrification [J]. Soil Biology and Biochemistry, 1998, 30(4): 433-442.
[23] CONRAD R. Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO) [J]. Microbiological Reviews, 1996, 60(4): 609-640.
[24] DAVIDSON E A, KELLER M, ERICKSON H E, et al. Testing a conceptual model of soil emissions of nitrous and nitric oxides [J]. Bioscience, 2000, 50(8): 667-680.
[25] 黄国宏,陈冠雄,韩冰. 土壤含水量与 N2O 产生途径研究 [J]. 应用生态学报,1999,10(1):53-56.