多年生稻稻瘟病抗性评价
收稿日期: 2020-11-23
网络出版日期: 2021-01-20
基金资助
云南省重大科技专项“多年生稻种质创新及应用研究”（2019ZG013）
Evaluation of Rice Blast Resistance in Perennial Rice
1st author: heyifei@mail.ynu.edu.cn
Received date: 2020-11-23
Online published: 2021-01-20
利用长雄野生稻（Oryza longistaminata）地下茎无性繁殖特性培育多年生稻（Perennial Rice, PR）已经成功并开始示范推广。多年生稻表现出一定的稻瘟病抗性,但其所具有的稻瘟病抗性来源尚不清楚。本研究通过田间病情调查、接种鉴定以及抗性基因检测等3种方法,对育成的多年生稻23（PR23）、云大24（PR24）、云大25（PR25）、云大101（PR101）、云大107（PR107）、父本长雄野生稻、母本RD23、（RD23/长雄野生稻）F1进行稻瘟病抗性评价。结果表明,父本长雄野生稻、（RD23/长雄野生稻）F1代及5个多年生稻品种（系）表现为高抗稻瘟病,而母本RD23表现高感稻瘟病,推测PR23、PR24、PR25、PR101和PR107这5个多年生稻品种（系）的稻瘟病抗性可能来源于长雄野生稻。其中,PR23、PR25稻瘟病抗性基因可能来自于长雄野生稻的Pi5基因和Pita-2位点,PR24稻瘟病抗性基因可能是来自长雄野生稻的Pita-2位点,PR107稻瘟病抗性基因可能来自于长雄野生稻的Pi5基因和Pish位点;PR101中未检测到本文中涉及到的基因或位点,推测其稻瘟病抗性来自长雄野生稻内未知的稻瘟病抗性基因。本研究结果将为多年生稻稻瘟病抗病育种、品种布局、植保技术制定等提供一定参考。
何奕霏, 秦世雯, 张石来, 黄光福, 张静, 杨勤忠, 胡凤益 . 多年生稻稻瘟病抗性评价[J]. 中国稻米, 2021 , 27(1) : 9 -13 . DOI: 10.3969/j.issn.1006-8082.2021.01.003
Perennial rice has been developed successful by utilizing the rhizome trait of the wild rice species, Oryza longistaminata and demonstrated widely, exhibiting broad-spectrum resistance to rice blast. To understand the reason of resistance to blast of perennial rice, disease investigation in famer's field, inoculation identification and molecular detection of rice blast resistant genes were carried out among nine accessions including five perennial rice varieties （lines） as Perennial Rice 23（PR23）, Yunda24 （PR24）, Yunda25 （PR25）, Yunda101 （PR101）, Yunda107 （PR107）, male parent O. longistaminata, female parent RD23, F1（RD23/O. longistaminata） and Lijiangxintuanheigu（LTH）. The results showed that all the five perennial rice varieties（lines）, their male parent O. longistaminata and the original F1（RD23/O. longistaminata） had high resistance to rice blast, while their female parent RD23 showed high susceptibility, which indicated that the rice blast resistance gene of these five perennial rice lines may originated from O. longistaminata. The resistance of PR23 and PR25 might owing to the Pi5 gene and Pita-2 locus from O. longistaminata, and the resistance of PR24 might due to the pita-2 locus from O. longistaminata, while that of PR107 might inherited from O. longistaminata with the Pi5 gene and Pish locus. However, none of the known rice blast resistance genes or loci mentioned in this study were harbored by PR101, speculated that the unknown genes from O. longistaminata inherited by PR101 archived its blast resistance. The results of this study provided a basis and strategy for blast disease resistance breeding, perennial rice varieties distribution and plant protection technology decision in future.
Key words： perennial rice; rice blast; resistance evaluation; Oryza longistaminata
| [1] | 谭秀芳,沈玮囡,任广乾,等. 水稻稻瘟病发生特点及防治措施研究进展[J]. 农业科技通讯,2018(7):237-239. |
| [2] | 曹妮,陈渊,季芝娟,等. 水稻抗稻瘟病分子机制研究进展[J]. 中国水稻科学,2019,33(6):489-498. |
| [3] | 华丽霞,何炼,蒋秋平,等. 稻瘟病抗性基因特异性分子标记的开发及应用进展[J]. 分子植物育种,2016,14(10):2 739-2 748. |
| [4] | TAO D, SRIPICHITT S.Preliminary report on transfer traits of vegetative propagation from wild rice species to oryza sativa via distant hybridization and embryo rescue[J]. The Kasetsart J, Natural Sci, 2000, 34: 1-11. |
| [5] | 胡凤益. 长雄野生稻(Oryza longistaminata)地下茎分子定位和遗传研究[D]. 重庆:西南农业大学,2002. |
| [6] | 胡凤益. 长雄野生稻地下茎基因Rhz2、Rhz3精细定位及地下茎全基因组表达谱分析[D]. 北京:中国农业科学院,2010. |
| [7] | HUANG G, QIN S, ZHANG S, et al.Performance, economics and potential impact of perennial rice PR23 relative to annual rice cultivars at multiple locations in Yunnan province of China[J]. Sustainability, 2018, 10:1086. |
| [8] | XU P, DONG L, ZHOU J, et al.Identification and mapping of a novel blast resistance gene Pi57(t) in Oryza longistaminata[J]. Euphytica, 2015, 205(1): 95-102 . |
| [9] | International Rice Research Institute (IRRI). Standard evaluation system for rice[S]. 2002. |
| [10] | 华丽霞,汪文娟,陈深,等. 抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发[J]. 中国水稻科学,2015,29(3):305-310. |
| [11] | 高利军,高汉亮,颜群,等. 4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J]. 杂交水稻,2010,25(suppl1):294-298. |
| [12] | RAMKUMAR G, SRINIVASARAO K, MOHAN K, et al.Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pikh)[J]. Mol Breed, 2011, 27(1): 129-135. |
| [13] | 刘开强,伍豪,颜群,等. 水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J]. 西南农业学报,2016,29(6):1 241-1 244. |
| [14] | COSTANZO S, JIA Y.Sequence variation at the rice blast resistance gene Pi-km locus: implications for the development of allele specific markers[J]. Plant Sci, 2010, 178(6): 523-530. |
| [15] | KOIDE Y, EBRON L, KATO H, et al.A set of near-isogenic lines for blast resistance genes with an indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background[J]. Field Crop Res, 2011, 123(1): 19-27. |
| [16] | LI W, LEI C, CHENG Z, et al.Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding[J]. Mol Breed, 2008, 22(1): 141-149. |
| [17] | 苏世杰,刘二明,李小娟,等. 稻瘟病菌致病性变异研究进展[J]. 江西植保,2006(3):111-115. |
| [18] | HE R, SALVATO F, PARK J, et al.A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement[J]. BMC Plant Biol, 2014, 14(1): 1-20. |
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