
优质籼稻品种黄华占和扬稻6号稻米理化特性和淀粉精细结构比较
收稿日期: 2020-10-20
网络出版日期: 2021-01-20
基金资助
国家自然科学基金(U19A2032;31825019);江苏省重点研发计划(现代农业)项目;江苏省高校优势学科工程资助项目
Comparative Analysis of Grain Physicochemical Properties and Starch Fine Structure between Indica Rice Cultivars Huanghuazhan and Yangdao 6 with Good Quality
Received date: 2020-10-20
Online published: 2021-01-20
以携带相同Wxb基因的籼稻品种黄华占和扬稻6号为试验材料,系统分析了这2个优质籼稻品种的理化特性和淀粉精细结构。结果表明,黄华占稻米蛋白质含量显著低于扬稻6号,而表观直链淀粉含量高于扬稻6号,其他理化品质如胶稠度、总淀粉含量和碱消值两者无显著差异;黄华占稻米具有较高的糊化温度和热焓值。黄华占和扬稻6号稻米淀粉粘滞曲线较为接近,但黄华占稻米具有较高的崩解值和较小的回复值。扫描电镜分析表明,淀粉粒形态和结构在2个样品间无明显差异;但淀粉精细结构分析表明,黄华占稻米淀粉含有较少的支链淀粉短链以及较多的支链长链和较多的直链淀粉短链;淀粉晶体结构分析表明,黄华占和扬稻6号淀粉均为A型晶体,但黄华占稻米淀粉的长程有序结构较扬稻6号淀粉高,而短程有序结构样品间无显著差异。上述研究为选育优质籼稻提供了参考。
陈飞, 张昌泉, 周少川, 李爱宏, 刘巧泉 . 优质籼稻品种黄华占和扬稻6号稻米理化特性和淀粉精细结构比较[J]. 中国稻米, 2021 , 27(1) : 14 -19 . DOI: 10.3969/j.issn.1006-8082.2021.01.004
With the high-quality indica rice varieties Huanghuazhan(HHZ) and Yangdao 6(9311) carrying the same Wxb allele as experimental materials, the grain physicochemical properties and starch fine structures of the two varieties were systematically analyzed. The results showed that the protein content of HHZ rice was significantly lower than 9311 rice, and the apparent amylose content was higher than that of 9311. There was no significant difference in other physicochemical properties between the two varieties such as gel consistency, total starch content and alkalinity. The thermal profiles of rice flours were analyzed and rice flours from HHZ exhibited higher gelatinization temperature and gelatinization enthalpy compared with 9311. The pasting properties from RVA analysis showed that both of HHZ and 9311 showed similar RVA curves, however, HHZ rice showed higher breakdown and lower setback than 9311 rice. The morphology of starch granule was observed by using scanning electron microscopy and there were no significant differences between the two varieties. Besides, the starch fine structure were investigated, and we found that HHZ grain starch contained reduced amylopectin short chains and increased longer amylopectin chains. Then the crystalline structure analysis showed that starch from HHZ presented higher level of long-range order structure than that of 9311 while both showed similar short-range order structure. The present data provides useful information for grain improvement in indica rice and also the further application of HHZ and 9311 in rice production.
| [1] | 张昌泉,赵冬生,李钱峰,等. 稻米品质性状基因的克隆与功能研究进展[J]. 中国农业科学,2016,49(22):4 267-4 283. |
| [2] | ZHOU H, XIA D, HE Y.Q. Rice grain quality-traditional traits for high quality rice and health-plus substances[J]. Molecular Breeding, 2020, 40:1-17. |
| [3] | LI H Y, PRAKASH S, NICHOLSON T, et al.The importance of amylose and amylopectin fine structure for textural properties of cooked rice grains[J]. Food Chemistry, 2016, 196: 702-711. |
| [4] | TAO K Y, LI C, YU W W, et al.How amylose molecular fine structure of rice starch affects functional properties[J]. Carbohydr Polym, 2019, 204: 24-31. |
| [5] | ZHANG C Q, ZHU J H, CHEN S J, et al.Wxlv, the ancestral allele of rice Waxy gene[J]. Molecular Plant, 2019, 12: 1 157-1 166. |
| [6] | HE X P, ZHU C L, LIU L L, et al.Difference of amylopectin structure among various rice genotypes differing in grain qualities and its relation to starch physicochemical properties[J]. Acta Agronomica Sinica, 2010, 36(2): 276-284. |
| [7] | 鄂志国,程本义,孙红伟,等. 近40年我国水稻育成品种分析[J]. 中国水稻科学,2019,33(6):523-531. |
| [8] | 林海,王志刚,鄂志国,等. 2018年我国审定的水稻品种基本特性分析[J]. 中国稻米,2019,25(6):65-71. |
| [9] | 吕凤,杨帆,范滔,等. 1977—2018年水稻品种审定数据分析[J]. 中国种业,2019(2):35-46. |
| [10] | 戴正元,赵步洪,刘晓静,等. 优质高产多抗中籼新品种扬稻6号[J]. 江苏农业科学,1997(4):13-14. |
| [11] | 白和盛,詹存钰,王宝和,等. 中籼扬稻6号及其在杂交稻育种中的应用[J]. 杂交水稻,2001(6):13-15. |
| [12] | 周少川,李宏,黄道强,等. 优质稻核心种质黄华占及其衍生系统理想模式研究[J]. 中国水稻科学,2009,23(2):45-51. |
| [13] | 周少川,李宏,黄道强,等. 国标一级优质稻品种黄华占的选育及应用[J]. 湖北农业科学,2012,51(10):1 960-1 964. |
| [14] | 黎彬,罗先富,朱建宇,等. 优质稻黄华占的特征特性及高产栽培技术[J]. 湖南农业科学,2009(6):31-32. |
| [15] | LITTLE R R, HILLER G B, SON E H D. Differential effect of dilute alkali on 25 varieties of milled white rice[J]. Cereal Chemistry, 1958, 35: 111-126. |
| [16] | ZHANG C Q, CHEN S J, REN X Y, et al.Molecular structure and physicochemical properties of starches from rice with different amylose contents resulting from modification of OsGBSSI activity[J]. Journal of Agricultural and Food Chemistry, 2017, 65(10): 2 222-2 232. |
| [17] | CAI J W, CAI C H, MAN J M, et al.Crystalline and structural properties of acid-modified lotus rhizome C-type starch[J]. Carbohydr Polym, 2014, 102: 799-807. |
| [18] | 舒庆尧,吴殿星,夏英武,等. 稻米淀粉RVA谱特征与食用品质的关系[J]. 中国农业科学,1998,31(3):25-29. |
| [19] | LI C, WU A, YU W W, et al.Parameterizing starch chain-length distributions for structure-property relations[J]. Carbohydr Polym, 2020, 241: 116 390. |
| [20] | CHEETHAM NWH, TAO L.Variation in crystalline type with amylose content in maize starch granules: an X-ray powder diffraction study[J]. Carbohydr Polym, 1998, 36: 277-284. |
| [21] | SEVENOU O, HILL S E, FARHAT I A, et al.Organisation of the external region of the starch granule as determined by infrared spectroscopy[J]. International Journal Biological Macromolecules, 2002, 31(1-3): 79-85. |
| [22] | TIAN Z, QIAN Q, LIU Q, et al.Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(51): 21 760-21 765. |
| [23] | 朱昌兰,沈文飚,翟虎渠,等. 水稻低直链淀粉含量基因育种利用的研究进展[J]. 中国农业科学,2004,37(2):157-162. |
| [24] | 赵春芳,岳红亮,黄双杰,等. 南粳系列水稻品种的食味品质与稻米理化特性[J]. 中国农业科学,2019,52(5):138-149. |
| [25] | XIE L, CHEN N, DUAN B, et al.Impact of proteins on pasting and cooking properties of waxy and non-waxy rice[J]. Journal Cereal Science, 2008, 47(2): 372-379. |
| [26] | ZHANG H, ZHOU L, XU H, et al.The qsAC3 locus from indica rice effectively increases amylose content under a variety of conditions[J]. BMC Plant Biology, 2019, 19(1): 275. |
| [27] | CHEN Z Z, LU Y, FENG L H, et al.Genetic dissection and functional differentiation of ALKa and ALKb, Two natural alleles of the ALK/SSIIa gene, responding to low gelatinization temperature in rice[J]. Rice, 2020, 13(1): 39. |
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