以常规粳稻秀水134为材料,设置清水浸种（CK）和咪酰胺浸种（Soak）两个处理,采用16S rRNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon 指数、Chao1 指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。

The effects of seed soaking agent prochloraz on the structure of microbial community in rice seeds were studied by setting seed soaking with water （CK） and seed soaking with prochloraz （Soak）, 16S rRNA amplicon sequencing and isolation culture methods in conventional japonica rice Xiushui 134. The results showed that compared with CK, the number of fungi on rice seed surface of Soak was significantly reduced. However, Soak had no significant effect on the diversity and composition of bacterial communities in rice seeds, including Shannon index, Chao1 index, Observed OTUs, β diversity and relative abundance of dominant bacteria. Thus, in agricultural production, prochloraz soaking as a measure to control rice seed-borne diseases has no significant effect on seed endophytic bacteria.