中国水稻百年育种的一些关键基因
收稿日期: 2021-10-18
网络出版日期: 2022-03-17
基金资助
国家科技重大专项(2018ZX08003-02B)
Key Genes in Century Rice Breeding in China
Received date: 2021-10-18
Online published: 2022-03-17
水稻育种就是培育具有新的或者改良性状的品种。而育种的本质,就是聚合有利基因或有利基因型集合。回顾我国百余年的水稻育种,每次跨越式突破,都是一些核心基因发挥了关键性作用。半矮秆基因sd1、野败细胞质雄性不育基因WA352及恢复基因Rf3和Rf4、光温敏核不育基因pms3或温敏核不育基因tms5的发掘和利用分别导致了中国水稻育种的三次跨越式突破——矮秆水稻的育成、三系法杂交水稻和两系法杂交水稻的发明。当前正在研究的一些关键基因, 例如Pi-n、Wx、ALK、Chalk5、fgr、S5-n在抗病、优质、籼粳亚种间杂种优势育种上也表现出良好结果。因此,作者预测将来的抗除草剂、抗虫、耐热、耐冷、抗倒、耐盐碱等育种必将由一些关键基因（家属）的发掘和利用所推动。
刘定富, 尹合兴, 应继锋 . 中国水稻百年育种的一些关键基因[J]. 中国稻米, 2022 , 28(2) : 1 -11 . DOI: 10.3969/j.issn. 1006- -8082.2022.02.001
The goal of rice breeding is to develop a new variety or to improve desired traits. The nature of the rice breeding is to integrate all the superior genes or genotypes and to develop a new variety/genotype. China’s modern rice breeding program was originated in the 1920’s and has gone through one-hundred-year history. Breaking-through of rice yield in each decade mostly are attributed to some well known key genes. Semidwarf gene sd1 played an important roles in developing high yield rice variety（eg IR8） and promoting the “green revolution” in south and south-east Asian. Wild abortion cytoplasmic male sterility gene WA352 and restoring gene Rf3/Rf4 played critical roles in three-line hybrid rice breeding technology, which resulted in the successful commercialization of the hybrid rice in China. The discovery, development, application of photo-thermo-sensitive genic male sterile gene pms3 and/or thermo-sensitive genic male sterile gene tms5 resulted in successful production of two-line hybrid rice breeding technology. The currently discovering and studying of Pi-n, Wx, ALK, Chalk5, fgr, S5-n in developing disease-resistent, better grain quality and intersubspecfic heterosis between Indica and Japonica hybrid breeding technology shows promising results in commercial rice production stage. We speculate that herbicide-resistant, insect-resistant, heat- or cold-tolerant, lodge-resistant, and saline-alkali-tolerant genes will play an important role in breeding next-generation rice variety.
| [1] | 应继锋, 刘定富, 赵健. 第5代(5G)作物育种技术体系[J]. 中国种业, 2020(10):1-3. |
| [2] | 刘定富, 赵健, 应继锋. 农作物育种的基本要点[J]. 中国稻米, 2020, 26(6):23-26. |
| [3] | 程式华. 中国水稻育种百年发展与展望[J]. 中国稻米, 2021, 27(4):1-6. |
| [4] | TAN L, LI X, LIU F, et al. Control of a key transition from prostrate to erect growth in rice domestication[J]. Nature Genetics, 2008, 40(11):1 360. |
| [5] | HUA L, WANG D R, TAN L, et al. LABA1, a domestication gene associated with long, barbed awns in wild rice[J]. Plant Cell, 2015, 27(7):1 875-1 888. |
| [6] | VARSHNEY R K, SINHA P, SINGH V K, et al. 5Gs for crop genetic improvement[J]. Current Opinion in Plant Biology, 2020, 56:190-196. |
| [7] | 程式华主编. 中国超级稻育种[M]. 北京: 科学出版社, 2010. |
| [8] | ZHANG G, BHARAJ T S, LU Y, et al. Mapping of the Rf3 nuclear fertility restoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers[J]. Theoretical and Applied Genetics, 1997, 94:27-33. |
| [9] | YAO F Y, XU C G, YU S B, et al. Mapping and genetic analysis of two fertility restorer loci the wide-abortive cytoplasmic male sterility system of rice (Oryza sative L.)[J]. Euphytica, 1997, 98:183-187. |
| [10] | 何光华, 王文明, 刘国庆, 等. 利用SSR标记定位明恢63的2对恢复基因[J]. 遗传学报, 2002(9):798-802. |
| [11] | 梁国华. 水稻三种细胞质雄性不育系的恢复性遗传研究[D]. 广州:华南农业大学, 1998. |
| [12] | TAN X L, VANAVI C A, AMORNSILPA S, et al. Genetic analysis of rice CMS-WA fertility restoration based on QTL mapping[J]. Theoretical and Applied Genetics, 1998, 97(5/6):994-999. |
| [13] | BAZRKAR L, ALI A J, BABAEIAN N A, et al. Tagging of four fertility restorer loci for wild abortive—cytoplasmic male sterility system in rice(Oryza sativa L.) using microsatellite markers[J]. Euphytica, 2008, 164(3):669-677. |
| [14] | 李子银, 林兴华, 谢岳峰, 等. 利用分子标记定位农垦58S的光敏核不育基因[J]. 植物学报, 1999(7):58-62. |
| [15] | 景润春, 何予卿, 黄青阳, 等. 水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析[J]. 中国农业科学, 2000, 33(2):6. |
| [16] | JING R C, LI X M, YI P, et al. Mapping fertility-restoring genes of rice WA cytoplasmic male sterility using SSLP markers[J]. Botanical Bulletin of Academia Sinica, 2001, 42(3):167-171. |
| [17] | AHMADIKHAH A, KARLOV G I. Molecular mapping of the fertility-restoration gene Rf4 for WA-cytoplasmic male sterility in rice[J]. Plant Breeding, 2010, 125(4):363-367. |
| [18] | 梅明华, 陈亮, 章志宏, 等. 农垦58S光敏不育基因突变位点的确定及pms3区间的进一步作图[J]. 中国科学(C辑), 1999, 29(3):6. |
| [19] | 李子银, 林兴华, 谢岳峰, 等. 利用分子标记定位农垦58S的光敏核不育基因[J]. 植物学报(英文版), 1999, 41(7):731-735. |
| [20] | 陈亮, 梅明华, 徐才国, 等. 鉴定与水稻光敏核不育基因pms3连锁的AFLP-RFLP标记[J]. 厦门大学学报(自然科学版), 2000, 39(4):421-425. |
| [21] | 李香花, 王伏林, 陆青, 等. 水稻光敏核不育基因pms3的精细定位[J]. 作物学报, 2002, 28(3):310-314. |
| [22] | LU Q, LI X H, GUO D, et al. Localization of pms3, a gene for photoperiod-sensitive genic male sterility, to a 28.4-kb DNA fragment[J]. Molecular Genetics and Genomics, 2005, 273(6):507-511. |
| [23] | 贾建航, 李传友, 邓启云, 等. 用AFLP标记快速构建遗传连锁图谱并定位一个新基因tms5[J]. 植物学报(英文版), 2003, 45(5):614-619. |
| [24] | WANG Y G, XING Q H, DENG Q Y, et al. Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5[J]. Theoretical and Applied Genetics, 2003, 107(5):917-921. |
| [25] | 姜大刚, 卢森, 周海, 等. 用EST和SSR标记定位水稻温敏不育基因tms5[J]. 科学通报, 2006, 51(2):148-151. |
| [26] | YANG Q, LIANG C, ZHUANG W, et al. Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping[J]. Planta, 2007, 225(2):321-330. |
| [27] | LIU G, LU G, ZENG L, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6[J]. Molecular Genetics & Genomics, 2002, 267(4):472-480. |
| [28] | QU S, LIU G, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9 encodes an NBS-LRR protein and is a member of a multigene family in rice[J]. Genetics, 2006, 172(3):1 901-1 914. |
| [29] | BRYAN G T. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. Plant Cell Online, 2000, 12(11):2 033-2 046. |
| [30] | 崔旭红, 焦晓国, 张国安, 等. 转Bt基因水稻对稻飞虱及蜘蛛种群数量的影响[J]. 华中农业大学学报, 2002, 21(4):356-358. |
| [31] | GAO Z Y, ZENG D L, CUI X, et al. Map-based cloning of the ALK gene, which controls the gelatinization temperature of rice[J]. China Science C Life, 2003, 46(6):661-668. |
| [32] | ZHANG G, CHENG Z, ZHANG X, et al. Double repression of soluble starch synthase genes SSIIa and SSIIIa in rice (Oryza sativa L.) uncovers interactive effects on the physicochemical properties of starch[J]. Genome, 2011, 54(6):448-459. |
| [33] | LI Y, FAN C, XING Y, et al. Chalk5 encodes a vacuolar H+-translocating pyrophosphatase influencing grain chalkiness in rice[J]. Nature Genetics, 2014, 46(4):398-404. |
| [34] | BRADBURY L, HENRY R J, JIN Q, et al. A perfect marker for fragrance genotyping in rice[J]. Molecular Breeding, 2005, 16(4):279-283. |
| [35] | WENG J, GU S, WAN X, et al. Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight[J]. Cell Research, 2008, 18(12):1 199-1 209. |
| [36] | LIU J, CHEN J, ZHENG X, et al. GW5 acts in the brassinosteroid signalling pathway to regulate grain width and weight in rice[J]. Nature Plants, 2017, 3:17-43. |
| [37] | MI J M, LEI Y, KIM S R, et al. An effective strategy for fertility improvement of indica-japonica hybrid rice by pyramiding S5-n, f5-n, and pf12-j[J]. Molecular Breeding, 2019, 39(9):1-13. |
| [38] | YAN H M, QIN R, JIN W W, et al. Comparative physical mapping of Bph3 with BAC-FISH in Oryza officinalis and O. sativa[J]. Acta Botanica Sinica, 2002, 44(5):583-587. |
| [39] | LI J, MENG X, ZONG Y, et al. Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9[J]. Nature Plants, 2016, doi: 10.1038/nplants.2016.139. |
| [40] | 戴燚, 赵德刚. 抗草甘膦水稻突变体osgr-1 EPSPS基因克隆及生物信息学分析[J]. 种子, 2018, 37(3):1-6. |
| [41] | 唐晓艳, 柳威, 王承旭, 等. 水稻抗除草剂蛋白及其在植物育种中的应用:中国,CN 102586215A[P]. 2012-07-18. |
| [42] | 张保龙, 陈天子, 凌溪铁, 等. ALS突变型基因及其蛋白在抗除草剂方面的应用：中国,CN 105779479 B[P]. 2017-07-06. |
| [43] | 夏兰琴, 孙永伟, 赵云德, 等. 利用 CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用：中国,CN 106811479 A[P]. 2017-06-09. |
| [44] | 周延彪, 秦鹏, 赵新辉, 等. 抗除草剂水稻种质资源研究进展[J]. 杂交水稻, 2019, 34(1):1-5. |
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