
粳稻种质资源稻瘟病抗性及抗性基因分析
收稿日期: 2021-11-03
网络出版日期: 2022-03-17
基金资助
河北省农林科学院农业科技创新专项“高产粳稻新理想株型创制与超级粳稻新品种选育”(2022KJCXZX-BHS-1);唐山市科技计划项目“优质水稻新品种选育及配套技术研究”(19150243E);唐山市科技计划项目“非转基因抗除草剂型优质、香味、高产水稻种质创新”(19150242E);长粒香型和抗除草剂水稻种质创新与新品种选育(21326305D-2);河北省现代农业产业技术体系小麦产业创新团队项目(HBCT2018 010208);河北省重点研发计划项目“耐盐优质高产水稻新品种选育与示范”(19226340D)
Analysis of Rice Blast Resistance and Resistance Genes in Japonica Rice Germplasm Resources
Received date: 2021-11-03
Online published: 2022-03-17
邹拓, 耿雷跃, 张薇, 杜琪, 孟令启, 张启星 . 粳稻种质资源稻瘟病抗性及抗性基因分析[J]. 中国稻米, 2022 , 28(2) : 45 -50 . DOI: 10.3969/j.issn.1006-8082.2022.02.009
In order to improve the breeding level of rice blast resistance in eastern Hebei Province, 157 japonica rice resources were identified by field-induced susceptibility for rice blast resistance, and 12 disease resistance gene markers were used for genotype identification. The results showed that among the 157 japonica rice resources, there were 2, 70, 64, 9 and 12 materials showing resistance, medium resistance, medium susceptibility, susceptibility, and high susceptibility, respectively. Among them, materials ZY1 and ZY2 showed disease resistance. The resistance genes Piz and Pid3 had the highest frequency, while Pigm, Pia, Pita, Pik and Pi5 played the main role in the resistance of rice blast in eastern Hebei Province, and the more genes aggregated, the higher the resistance. Combining the agronomic traits and yield traits of germplasm resources, it is found that ZY2, ZY13, ZY15 and ZY21 are suitable as parents for rice blast resistance breeding.
Key words: rice; rice blast resistance evaluation; resistance genes
| [1] | PADMANABHAN S Y. Estimating losses from rice blast in India[M]// International Rice Research Institute (IRRI):The rice Blast Disease. Baltimore, Maryland: Johns Hopkins Press, 1965: 203-221. |
| [2] | KHUSH G S, JENA K K. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.)[M]// Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer Netherlands, 2009: 1-10. |
| [3] | WANG C, WEN G, LIN X, et al. Identification and fine mapping of the new bacterial blight resistance gene, Xa31(t), in rice[J]. European Journal of Plant Pathology, 2009, 123(2):235-240. |
| [4] | CORREA VICTORIA F J, MARTINEZ RACINES C P. Genetic structure and virulence diversity of Pyricularia grisea for breeding for rice blast resistance[J]. Arterioscler Thromb Vasc Biol, 1995, 15(2):219-227. |
| [5] | SMITH P J. The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines[J]. Phytopathology, 2009, 99(3):243-250. |
| [6] | ZEIGLER R S, CUOC L X, SCOTT R P, et al. The relationship between lineage and virulence in Pyricularia grisea in the Philippines[J]. Phytopathology, 1995, 85(4):443-451. |
| [7] | PARKER D, BECKMANN M, ENOT D P, et al. Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions[J]. Nature Protocols, 2008, 3(3):435-445. |
| [8] | MOFFAT A S. Plant genetics. mapping the sequence of disease resistance[J]. Science, 1994, 265(5180):1 804-1 805. |
| [9] | 朱小丽, 陶跃之, 周波. 水稻抗稻瘟病基因的复等位分布特征及其在基因挖掘中的应用[J]. 浙江农业学报, 2015, 27(10):1 789-1 796. |
| [10] | 徐小金. 稻瘟病抗性基因资源的挖掘及其育种利用[D]. 金华:浙江师范大学, 2016. |
| [11] | 刘洋, 徐培洲, 张红宇, 等. 水稻抗稻瘟病Pib基因的分子标记辅助选择与应用[J]. 中国农业科学, 2008, 41(1):9-14. |
| [12] | 王忠华, 贾育林, 吴殿星, 等. 水稻抗稻瘟病基因Pi-ta的分子标记辅助选择[J]. 作物学报, 2004, 30(12):1 259-1 265. |
| [13] | 曾晓珊, 杨先锋, 赵正洪, 等. 稻瘟病抗病基因Pia的抗性分析及精细定位[J]. 中国科学:生命科学, 2011, 41(1):70-77. |
| [14] | 全国农业技术推广服务中心, 浙江省农业科学院植物保护与微生物研究所, 广东省农业科学院植物保护研究所, 等. NY/T 2646- 2014.水稻品种试验稻瘟病抗性鉴定与评价技术规程[S]. 北京: 中国标准出版社, 2014. |
| [15] | 孙庚. 水稻抗瘟基因与稻瘟菌无毒基因的分布及稻瘟菌分子检测[D]. 长春:吉林大学, 2012. |
| [16] | TIAN D G, CHEN Z J, CHEN Z Q, et al. Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars[J]. Rice, 2016, 9(1):1-11. |
| [17] | 梁毅, 杨婷婷, 谭令辞, 等. 水稻广谱抗瘟基因Pigm紧密连锁分子标记开发及其育种应用[J]. 杂交水稻, 2013, 28(4):63-68. |
| [18] | HAYASHI K, YOSHIDA H, ASHIKAWA I. Development of PCR-based allele specific and InDel marker sets for nine rice blast resistance genes[J]. Theoretical and Applied Genetics, 2006, 113(1):251-260. |
| [19] | OKUYAMA Y, KANZAKI H, ABE A, et al. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes[J]. Plant Journal, 2011, 66(3):467-479. |
| [20] | SHARMA T R, MADHAV M S, SINGH B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea[J]. Molecular Genetics and Genomics, 2005, 274(3):569-578. |
| [21] | 蔡海亚, 周雷, 焦春海, 等. 水稻抗稻瘟病基因Pita特异性分子标记开发及应用[J]. 分子植物育种, 2017, 15(2):589-593. |
| [22] | 田大刚, 苏军, 陈建民, 等. 1 092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J]. 分子植物育种, 2012, 10(2):214-221. |
| [23] | 李旭升, 向小娇, 申聪聪, 等. 水稻重测序核心种质资源的稻瘟病抗性鉴定与评价[J]. 作物学报, 2017, 43(6):795-810. |
| [24] | ASHKANI S, RAFII M Y, SHABANIMOFRAD M, et al. Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): Current status and future considerations[J]. Critical Reviews in Biotechnology, 2016, 36(2):353-367. |
| [25] | 张羽, 冯志峰, 张先平, 等. 5个稻瘟病抗性基因的基因型分型与抗性评价研究[J]. 西南农业学报, 2014, 27(5):1 929-1 936. |
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