专论与研究

亚精胺对盐胁迫下黄华占水稻幼苗根系抗氧化酶活性及Na+稳态的影响

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  • 1.广东海洋大学 滨海农业学院,广东 湛江 524088
    2.国家耐盐碱水稻技术创新中心 华南中心,广东 湛江 524088
*

收稿日期: 2022-09-08

  网络出版日期: 2023-03-14

基金资助

广东海洋大学国家级创新创业训练计划项目(CXXL2020013);广东海洋大学科研启动费资助项目(R20062);广东省重点领域研发计划项目(2020B020219004)

Effects of Spermidine on Antioxidant Enzyme Activity and Na+ Homeostasis of Seedlings Roots of Huanghuazhan Rice under Salt Stress

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  • 1. College of Coastal Agricultural Science, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China
    2. South China Branch of National Saline Alkali Tolerant Rice Technology Innovation Center, Zhanjiang, Guangdong 524088, China
*

Received date: 2022-09-08

  Online published: 2023-03-14

摘要

以黄华占种子为材料,研究盐胁迫条件下,外源亚精胺(Spd)对水稻幼苗根系的生长、抗氧化酶活性、活性氧(ROS)代谢、丙二醛(MDA)以及Na+稳态的影响。结果表明,经100 mmol/L NaCl溶液处理水稻幼苗根系的ROS、·O2-、H2O2、Na+和MDA含量显著升高,过氧化物酶(POD)和过氧化氢酶(CAT)活性显著降低;在盐胁迫条件下,施用0.1 mmol/L Spd显著提高水稻幼苗根系的CAT和POD活性,显著降低ROS、·O2-、H2O2、Na+和MDA含量,从而减轻Na+毒害和过氧化造成的膜损伤,保护生物膜系统的稳定,维持细胞的稳态。

本文引用格式

黄婷婷, 郑殿峰, 冯乃杰, 赵黎明, 周鸿凯, 沈雪峰 . 亚精胺对盐胁迫下黄华占水稻幼苗根系抗氧化酶活性及Na+稳态的影响[J]. 中国稻米, 2023 , 29(2) : 43 -47 . DOI: 10.3969/j.issn.1006-8082.2023.02.009

Abstract

The effects of exogenous spermidine (Spd) on rice seedling growth, antioxidant enzyme activity, reactive oxygen species (ROS) metabolism, malonic dialdehyde (MDA) and Na+ homeostasis were studied with rice variety Huanghuazhan as the material. The results showed that the content of ROS、·O2-、H2O2、Na+ and MDA were significantly increased in the root of rice seedlings by treated with 100 mmol·L-1 NaCl, meanwhile, the activities of catalase (CAT) and peroxidase (POD) were remarkably reduced. However, the activities of CAT and POD were significantly enhanced by using 0.1 mmol·L-1 Spd, and the content of ROS、·O2-、H2O2、Na+ and MDA were remarkably decreased. So, the damage of Na+ toxicity and membrane oxidation were alleviated, the stability of biofilm system was protected, and the steady state of cells was maintained with Spd under salt stress condition.

参考文献

[1] 杨劲松, 姚荣江, 王相平, 等. 中国盐渍土研究:历程、现状与展望[J]. 土壤学报, 2022, 59(1):10-27.
[2] 侯红燕, 董晓亮, 周红, 等. 滨海盐碱地不同氮肥用量对水稻干物质转运及稻米品质的影响[J]. 中国稻米, 2021, 27(1):27-31.
[3] ZHANG X X, SHI Z Q, TIAN Y J, et al. Salt stress increases content and size of glutenin macropolymers in wheat grain[J]. Food Chemistry, 2016, 197: 516-521.
[4] 王洋, 张瑞, 刘永昊, 等. 水稻对盐胁迫的响应及耐盐机理研究进展[J]. 中国水稻科学, 2022, 36(2):105-117.
[5] 王盼盼. NaCl胁迫下海水稻原生境种质HD961苗期生理及代谢组学分析[D]. 湛江: 广东海洋大学, 2021.
[6] 刘玲, 冯乃杰, 郑殿峰, 等. 叶喷海藻酸钠寡糖对盐胁迫下水稻幼苗抗逆性及生理特性的影响[J]. 生态学杂志, 2022,doi: 10.13292/j.1000-4890.202209.013.
[7] 李瑶, 郑殿峰, 冯乃杰, 等. 调环酸钙对盐胁迫下水稻幼苗生长及抗性生理的影响[J]. 植物生理学报, 2021, 57(10):1897-1 906.
[8] 张灵, 陶亚军, 方琳, 等. 植物多胺的代谢与生理研究进展[J]. 植物生理学报, 2020, 56(10):2029-2 039.
[9] 王燕, 刘青, 华春, 等. 外源亚精胺对盐胁迫下水稻根系抗氧化酶活性的影响[J]. 西北农业学报, 2009, 18(6):161-165.
[10] 海霞, 米俊珍, 赵宝平, 等. 外源亚精胺对盐胁迫下燕麦幼苗生长及生理特性的影响[J]. 西北植物学报, 2021, 41(6):1003-1 011.
[11] 侯君杰. 外源亚精胺缓解盐胁迫对水稻光合性能影响的机理研究[D]. 南京: 南京师范大学, 2021.
[12] 邹芳, 王志恒, 杨秀柳, 等. 外源亚精胺对盐胁迫下甜高粱(Sorghum bicolor)幼苗的影响[J]. 分子植物育种, 2020, 18(8):2721-2 727.
[13] CHEN Y, MO H Z, ZHENG M Y, et al. Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa[J]. PLoS One, 2014, 9: e110904.
[14] WANG T T, SHI Z Q, HU L B, et al. Thymol ameliorates cadmium-induced phytotoxicity in the root of rice (Oryza sativa) seedling by decreasing endogenous nitric oxide generation[J]. Journal of Agricultural and Food Chemistry, 2017, 65: 7 396-7 405.
[15] 黄洁, 黄晶, 梁青铎, 等. 盐胁迫对粳稻品种生长和生理特性的影响[J]. 中国稻米, 2021, 27(3):37-40.
[16] 刘丹, 刘美艳, 孙健, 等. 亚精胺提高玉米幼苗抗寒性的研究[J]. 玉米科学, 2021, 29(4):56-61.
[17] 徐芬芬, 韦蓉香. NAA对盐胁迫下水稻根系生长的缓解效应[J]. 杂交水稻, 2020, 35(2):75-77.
[18] 姚栋萍, 吴俊, 胡忠孝, 等. 水稻耐盐碱的生理机制及育种策略[J]. 杂交水稻, 2019, 34(4):1-7.
[19] DEINLEIN U, STEPHAN A B, HORIE T, et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science, 2014, 19: 371-379.
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