
超声波处理种子对香稻秧苗素质、抗逆性、产量及香气的影响
收稿日期: 2023-07-30
网络出版日期: 2023-09-15
基金资助
国家自然科学基金(31971843);广东省现代农业产业技术体系(2020KJ105);广州市科技计划项目(202103000075)
Effects of Treating Seeds with Ultrasound on Seedling Quality, Stress Resistance, Yield and Aroma of Fragrant Rice
Received date: 2023-07-30
Online published: 2023-09-15
以杂交香稻品种青香优19香为材料,设置超声波处理种子(UT,种子发芽前,放入隧道式超声波处理器,在室温下以50 kHz频率处理1.0 min)及非超声波处理种子(CK),用钵苗盘在低温人工气候箱(白天12 h 22 ℃,晚上12 h 12 ℃)、高温人工气候箱(白天12 h 35 ℃,晚上12 h 25 ℃)和室温网室(白天平均温度为25.3 ℃,夜间平均温度为16.6 ℃)培育秧苗,20 d后统一转移至网室盆中种植至成熟,测定苗期秧苗素质、抗逆性相关酶活性、产量和2-AP含量,以探明超声波处理种子对香稻秧苗素质、抗逆性、产量和香气的影响。结果表明,与CK相比,UT处理提高了水稻苗期植株的株高和茎基宽,显著提高了苗期植株的鲜质量和干质量;UT处理后青香优19香的POD、CAT活性和MDA含量与CK相比无显著差异,但SOD活性、结实率和每穗粒数显著提高,从而显著提高产量;UT处理种子显著提高了青香优19香的2-AP含量。
张莹莹, 何珍珍, 兰儒剑, 肖立中, 钟克友, 田华, 段美洋, 潘圣刚, 王树丽, 莫钊文, 唐湘如 . 超声波处理种子对香稻秧苗素质、抗逆性、产量及香气的影响[J]. 中国稻米, 2023 , 29(5) : 62 -65 . DOI: 10.3969/j.issn.1006-8082.2023.05.011
The effects of treating seed with ultrasound on seedling quality, stress resistance, yield, and aroma of fragrant rice were studied using fragrant rice variety Qingxiangyou 19 xiang as the material. Two treatments were set up for the experient: ultrasound treatment (UT, before germination, the seeds placed in a tunneled ultrasonic processor, treated at 50 kHz frequency for 1.0 min at room temperature) and non-ultrasound treatment (CK). The seedling were grown under different temperature conditions: low-temperature artificial climate chamber (12 hours of 22 ℃ during the day and 12 hours of 12 ℃ at night), high-temperature artificial climate chamber (12 hours of 35 ℃ during the day and 12 hours of 25 ℃ at night), and ambient temperature greenhouse (average daytime temperature of 25.3 ℃ and average nighttime temperature of 16.6 ℃). After 20 days of cultivation in seedling trays, the seedlings were transferred to pots in the greenhouse until maturity. The results showed that capared to CK, UT treatment increased the plant height and stem base width of rice seedlings during the seedling stage and significantly improved the fresh weight and dry weight of the seedlings. Compared to CK, the activities of peroxidase(POD), catalase(CAT), and the malondialdehyde(MDA) content in Qingxiangyou 19 xiang treated with UT were not shown significant differences but the superoxide dismutase(SOD) activity was significantly increased. UT treatment significantly increased the grain setting rate and the number of grains per panicle, resulting in a significant increase in yield. UT also significantly increased the 2-acetyl-1-pyrroline (2-AP) content in Qingxiangyou 19 xiang.
Key words: fragrant rice; ultrasound; seedling quality; stress resistance; yield; 2-acetyl-1-pyrroline
| [1] | 陈印军, 易小燕, 方琳娜, 等. 中国耕地资源与粮食增产潜力分析[J]. 中国农业科学, 2016, 49(6):1117-1 131. |
| [2] | 徐春春, 纪龙, 陈中督, 等. 2021年我国水稻产业形势分析及2022年展望[J]. 中国稻米, 2022, 28(2):16-19. |
| [3] | 匡勇, 郑华斌, 黄璜. 温度变化对湖南水稻产量的影响[J]. 作物研究, 2011, 25(6):538-543. |
| [4] | SASEENDRAN S A, SINGH K K, RATHORE L S. Effects of climate change on rice production in the tropical humidcimate of Kerala, India[J]. Climatic Change, 2000, 44: 495-514. |
| [5] | 冯世座, 刘合芹. 极端温度对籼粳稻苗期光合特性及生理生化特性的影响[J]. 浙江师范大学学报(自然科学版), 2012, 35(3):330-337. |
| [6] | DA SILVA J A T, DOBRANSZKI J. Sonication and ultrasound: Impact on plant growth and development.[J]. Plant Cell, Tissue and Organ Culture, 2017, 117(2): 131-143. |
| [7] | 黄卓烈, 陈小丽, 巫光宏, 等. 超声波对胰蛋白酶活力影响的机理研究[J]. 中国生化药物杂志, 2009, 30(4):230-234. |
| [8] | 聂俊, 严卓晟, 肖立中, 等. 超声波处理对水稻发芽特性及产量和品质的影响[J]. 广东农业科学, 2013, 40(1):13-15. |
| [9] | 傅友强, 刘彦卓, 梁开明, 等. 超声波处理对华南双季晚稻产量和稻米品质的影响[J]. 农学学报, 2020, 10(6):1-4. |
| [10] | 刘琪, 唐湘如, 潘圣刚, 等. 超声波处理种子和基肥施锌对香稻产量、糙米香气的影响[J]. 西南农业学报, 2014, 27(4):1551-1 555. |
| [11] | MO Z W, LIU Q, XIE W. Ultrasonic seed treatment and cu application modulate photosynthesis, grain quality, and cu concentrations in aromatic rice[J]. Photosynthetica, 2020, 58(3): 682-691. |
| [12] | 赖日芳, 郑阿香, 罗昊文, 等. 不同育秧方式对机插香稻秧苗素质及生理特性的影响[J]. 作物杂志, 2020(3):137-141. |
| [13] | MO Z W, LI Y H, NIE J, et al. Nitrogen application and different water regimes at booting stage improved yield and 2-acetyl-1-pyrroline (2AP) formation in fragrant rice[J]. Rice, 2019, 12(1): 74. |
| [14] | 万泗梅. 50 kHz超声波干法处理对水稻种子发芽、出苗及产量的影响[J]. 福建农业科技, 2018(6):12-15. |
| [15] | 黎国喜, 严卓晟, 闫涛, 等. 超声波刺激对水稻的种子萌发及其产量和品质的影响[J]. 中国农学通报, 2010, 26(7):108-111. |
| [16] | 李志, 朱有亮. 超声波处理水稻种子栽培示范[J]. 北方水稻, 2020, 50(2):42-43. |
| [17] | KONG L L, ASHRAF U, CHENG S R, et al. Short-term water management at early filling stage improves early-season rice performance under high temperature stress in south China[J]. European Journal of Agronomy, 2017, 90: 117-126. |
| [18] | 胡伟凤. 超声波处理对水稻种子活力及水稻幼苗抗冷性的影响[D]. 长沙: 湖南农业大学, 2018. |
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