“禾”是适应贵州黔东南山区峡谷森林中遮荫、泥沼、寒冷、积水等特殊自然生境生长的地方水稻品种。以402份禾种质资源为材料，依据禾颖尖颜色差异将原始种质划分为5组，基于26个表型性状，采用逐步聚类法以6种总体取样规模、4种组内取样比例和2种组内取样方法筛选最佳取样策略，构建禾初级核心种质，并对其进行综合评价。结果表明，10%为最适总体取样规模，组内取样比例法以对数比例法最优，聚类取样法是组内最佳取样方法。在最佳取样策略下，共获得47份禾初级核心种质，占比11.69%，表型保留比例为95.77%。核心种质评价结果表明，构建的核心种质作为原始种质的浓缩能够代表禾原始种质的遗传多样性。

‘HE’ is a rice landraces that adapts to the special natural habitat of shading, slough, cold and waterlogged in deep-valley and forests in the Qiandongnan mountainous areas in Guizhou. In this study, 402 HE resources, divided into 5 groups based on the color differences of the glume tip, were used as materials. Based on the 26 phenotypic traits, the progressive clustering method was applied to screen out the optional sampling strategy by using 6 overall sampling scales, 4 intra-group sampling ratios and 2 intra-group sampling methods, and to construct the primary core collection and evaluate it comprehensively. The results showed that 10% was the optimal overall sampling scale, the optimal intra-group sampling ratio method was the logarithmic ratio method, and the best intra-group sampling method was the cluster sampling method. Under the optimal sampling strategy, 47 primary core collections of HE was obtained, accounting for 11.69% of the whole germplasm, and the retention rate of phenotype was 95.77%. The core collection constructed in this study could be a good representation of the genetic diversity of the original germplasm as a concentration of it.