一种抗稻曲病菌的摩氏假单胞菌JP2-207及其抗性机制初探
收稿日期: 2023-11-24
网络出版日期: 2024-03-18
基金资助
浙江省重点研发计划项目(2021C02006);中央级公益性科研院所基本科研业务费(CPSIBRF-CNRRI-202115)
A Preliminary Study on the Mechanism of Pseudomonas mosselii JP2-207 Against Rice False Smut Fungus Ustilaginoidea virens
Received date: 2023-11-24
Online published: 2024-03-18
从水稻根际表面土壤分离到一株能有效抑制稻曲病菌（Ustilaginoidea virens）生长的细菌菌株JP2-207。16s rRNA基因片段扩增及系统发育树分析表明，JP2-207属于摩氏假单胞菌（Pseudomonas mosselii）。测序分析显示，JP2-207全基因组为1个大小5 702 443 bp的环状DNA，包含5 154个蛋白编码基因，22个rRNA和78个tRNA基因。生物信息学分析预测了9个与次生代谢产物生物合成相关的基因簇，其中多个基因簇产生的代谢物被报道对植物病原菌具有抑制活性。本研究为摩氏假单胞菌JP2-207及其代谢产物防治水稻稻曲病的研究奠定了初步的理论基础。
刘思慧, 明珂, 陈国庆, 冯国忠 . 一种抗稻曲病菌的摩氏假单胞菌JP2-207及其抗性机制初探[J]. 中国稻米, 2024 , 30(2) : 13 -17 . DOI: 10.3969/j.issn.1006-8082.2024.02.003
Pseudomonas mosselii JP2-207 was isolated from the soil of the rhizosphere of rice plants, this strain showed obvious inhibitory activity against Ustilaginoidea virens, an important rice pathogen. The complete genome of P. mosselii JP2-207 consists of 5 702 443 bp with a circle chromosome, 5 154 protein-coding genes, 22 rRNA genes, and 78 tRNA. The genomic analysis revealed that 9 candidate gene clusters were involved in the biosynthesis of secondary metabolites. Together, this study provides preliminary results for exploring the antagonistic mechanism of rice false smut by P. mosselii JP2-207 and its secondary metabolites.
Key words： Pseudomonas mosselii; rice; rice false smut disease; metabolite; gene clusters; biocontrol
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