专论与研究

苗期喷施茉莉酸甲酯对香稻品种象牙香占香气的影响

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  • 华南农业大学 亚热带农业生物资源保护与利用国家重点实验室/农业农村部华南地区作物栽培科学观测实验站/广州市丝苗香米科学与技术重点实验室,广州510642

第一作者:18843184689@163.com

收稿日期: 2023-10-29

  网络出版日期: 2024-05-20

基金资助

国家自然科学基金(31971843);广东省现代农业产业技术体系(2020KJ105);广州市科技计划项目(202103000075)

Effects of Spraying Methyl Jasmonate at Seedling Stage on Aroma of Fragrant Rice Xiangyaxiangzhan

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  • State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Bioresources, South China Agricultural University/Scientific Observing and Experimental Station of Crop Cultivation in South China, Ministry of Agriculture and Rural Affairs/Guangzhou Key Laboratory for Science and Technology of Aromatic Rice, Guangzhou 510642, China

1st author: 18843184689@163.com

Received date: 2023-10-29

  Online published: 2024-05-20

摘要

茉莉酸甲酯能够提高植物的抗逆境胁迫能力,但在对香稻香气物质2-乙酰基-1-吡咯啉(2-AP)的影响研究还鲜有报道。以香稻品种象牙香占为试验材料,设置茉莉酸甲酯3种喷施浓度(0.25、0.50和1.00 mmol/L),研究苗期喷施对香稻幼苗2-AP合成与累积、香气前体物质含量、香气相关酶活性、香气相关基因表达量调控、抗性酶活性变化和秧苗素质的影响。结果表明,与对照(CK)相比,喷施适当的茉莉酸甲酯浓度(0.50~1.00 mmol/L),香稻幼苗2-AP含量增加,香气合成前体物质1-吡咯啉、丙酮醛和1-吡咯啉-5-羧酸(P5C)含量提高,二胺氧化酶2基因(DAO4)、鸟氨酸转氨酶基因(OAT)和脯氨酸脱氢酶基因(ProDH)表达量提升。茉莉酸甲酯处理还提高了香稻秧苗素质,增加了抗性酶活性,提高了抗氧化能力。适度的茉莉酸甲酯能提高香稻2-AP含量和秧苗素质,归因于相关酶活性和基因表达量的提高。

本文引用格式

邢丕鹏, 何珍珍, 张震, 罗昊文, 张莹莹, 邓思呈, 莫钊文, 潘圣刚, 田华, 段美洋, 祁剑英, 唐湘如 . 苗期喷施茉莉酸甲酯对香稻品种象牙香占香气的影响[J]. 中国稻米, 2024 , 30(3) : 67 -71 . DOI: 10.3969/j.issn.1006-8082.2024.03.011

Abstract

Methyl jasmonate is known to enhance plant stress resistance, however, its specific effects on the accumulation of 2-acetyl-1-pyrroline (2-AP) in fragrant rice have not been extensively investigated. In this experiment, Xiangyaxiangzhan, a fragrant rice variety, was selected as the test material. Three concentrations of methyl jasmonate (0.25, 0.50, and 1.00 mmol/L) were applied during the seedling stage to examine the impact on the synthesis and accumulation of 2-AP, as well as the levels of aroma precursors, activity of aroma-related enzymes, regulation of aroma-related gene expression, changes in enzyme activities related to stress resistance, and seedling quality. The results showed that compared to the control (CK), spraying appropriate concentrations of methyl jasmonate (0.50-1.00 mmol/L) increased expression of DAO4, OAT, and ProDH in fragrant rice seedlings, accompanied by an elevation in 2-AP content. Furthermore, spraying methyl jasmonate increased the levels of aroma synthesis precursors including 1-pyrroline, methylglyoxal, and P5C. This resulted in an improvement in the quality of fragrant rice seedlings, an increase in the activity of stress-resistant enzymes, and enhanced antioxidant capacity.

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