为提高2-乙酰-1-吡咯啉（2-AP）检测的效率和准确性，试验选用4个香稻品种为材料，利用气相色谱质谱联用仪（GC-MS）技术检测了不同储藏、加工及萃取方式下香稻2-AP含量。试验设置了4个储藏温度（25 ℃、4 ℃、-20 ℃、-80 ℃）、4个碾磨时长（15 s、30 s、60 s、90 s）以及18种萃取方式（超声萃取、70 ℃水浴加热萃取、超声+70 ℃水浴加热萃取，萃取时间为0.5、1.0、2.0、3.0、4.0、5.0 h）。结果表明，在-20 ℃和-80 ℃条件下储藏的香稻2-AP含量显著高于其他储藏温度下储藏的香稻，这两个储藏温度之间无显著差异。碾磨时长显著影响2-AP含量，碾磨30 s时2-AP含量最低，60 s时2-AP含量达到较高水平。超声萃取和70 ℃水浴加热萃取在萃取4 h时效果最佳，超过此时长2-AP含量开始下降。因此，为确保香稻中2-AP检测的可靠性和效率，建议采取以下措施：香稻成熟后应将样品储藏在-20 ℃条件下，优先选择超声萃取或70 ℃水浴加热萃取方法，并控制研磨时长为60 s，以最大程度减少2-AP的损失。

To improve the efficiency and accuracy of 2-acetyl-1-pyrroline(2-AP) detection, the experiment selected 4 fragrant rice varieties as materials and used gas chromatography-mass spectrometry (GC-MS) technology to detect the content of 2-AP in fragrant rice under different storage, processing, and extraction methods. The experiment set up 4 storage temperatures (25 ℃, 4 ℃, -20 ℃, -80 ℃), 4 milling times (15 s, 30 s, 60 s, 90 s), and 18 extraction methods (ultrasonic extraction, 70 ℃ water bath heating extraction, ultrasonic+70 ℃ water bath heating extraction, with extraction times of 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 h). The results showed that the content of 2-AP in fragrant rice stored at -20 ℃ and -80 ℃ was significantly higher than that stored at other temperatures, with no significant difference between -20 ℃ and -80 ℃ storage temperatures. The Milling time significantly affected the 2-AP content in fragrant rice, with the lowest 2-AP content observed after 30 seconds of milling and a high level reached at 60 seconds. Ultrasonic extraction and 70 ℃ water bath heating extraction showed the best effect after 4 hours of extraction, after which the 2-AP content began to decrease. To ensure accuracy and efficient detection of 2-AP in fragrant rice, the following measures are recommended: store fragrat rice samples at -20 ℃ after maturation, prioritize ultrasonic extraction or 70 ℃ water bath extraction, and control milling time to 60 s to minimize the loss of 2-AP.