为筛选生物菌肥在水稻生产中的适宜施用模式，本研究以晚粳稻品种上师大19为材料，通过大田小区试验，系统探究不同生物菌肥施用方式对水稻产量、氮素积累与转运及氮肥利用率的影响。试验设6个处理：T1，不施氮对照；T2，常规施氮，纯N 225 kg/hm²；T3，T2+秧苗2叶1心期叶面喷施菌肥3.75 L/hm²；T4，T2+秧苗2叶1心期叶面喷施菌肥7.5 L/hm²；T5，T2+移栽前根系蘸根施用菌肥3.75 L/hm²；T6，T2+移栽前根系蘸根施用菌肥7.5 L/hm²。结果表明，与T2处理相比，配施生物菌肥可显著提高水稻产量。其中，T6处理产量最高，达9 858.0 kg/hm²，较T2处理显著增产13.74%，且其每穗粒数（97.1粒）显著高于其他处理；T6处理还能显著提升分蘖盛期茎叶氮含量与氮累积量、齐穗期穗氮含量、成熟期穗氮累积量及整株氮累积量，并有效降低茎叶氮滞留率；在氮肥利用率方面，T6处理的氮回收利用率（47.40%）、氮农学利用率（8.34 kg/kg）和氮肥偏生产力（43.81 kg/kg）较T2处理分别显著提高64.01%、171.66%和13.73%。综上，在常规施氮基础上，于水稻移栽前加施7.5 L/hm²（稀释5倍）生物菌肥蘸根处理，可有效促进生育前期氮素积累及其向穗部的转运，协同提升水稻产量与氮素利用效率，该模式适宜作为水稻绿色生产的生物菌肥施用技术推广应用。

To screen suitable application modes of bio-fertilizer in rice production, this study used the late japonica rice cultivar Shanghaishida 19 as the test material and conducted field plot experiments to systematically investigate the effects of different bio-fertilizer application methods on rice yield, nitrogen accumulation and translocation, and nitrogen use efficiency(NUE). Six fertilization treatments were designed: T1, no nitrogen control; T2, conventional nitrogen application (pure N 225 kg/hm²); T3, T2 + foliar spraying of bio-fertilizer at 3.75 L/hm² at the 2-leaf-1-heart stage of seedlings; T4, T2 + foliar spraying of bio-fertilizer at 7.5 L/hm² at the 2-leaf-1-heart stage of seedlings; T5, T2 + root-dipping with bio-fertilizer at 3.75 L/hm² before transplanting; and T6, T2 + root-dipping with bio-fertilizer at 7.5 L/hm² before transplanting. The results showed that, compared with T2, co-application of bio-fertilizer significantly increased rice yield. Among these, treatment T6 achieved the highest yield, reaching 9 858.0 kg/hm², representing a significant increase of 13.74% over T2, and its grains per panicle (97.1 grains) were significantly higher than those of other treatments (P<0.05). T6 also significantly increased stem-leaf nitrogen content and nitrogen accumulation at the tillering peak stage, panicle nitrogen content at the full heading stage, panicle nitrogen accumulation at maturity, and total plant nitrogen accumulation, while effectively reducing stem-leaf nitrogen retention rate. Regarding nitrogen use efficiency, the nitrogen recovery efficiency (47.40%), nitrogen agronomic efficiency (8.34 kg/kg), and nitrogen partial factor productivity (43.81 kg/kg) of T6 were significantly higher than those of T2 by 64.01%, 171.66%, and 13.73%, respectively. In conclusion, based on conventional nitrogen application, root-dipping with bio-fertilizer at 7.5 L/hm² (diluted 5 times) before rice transplanting can effectively promote nitrogen accumulation in the early growth stage and its translocation to panicles, synergistically enhance rice yield and nitrogen use efficiency. This model is suitable as a bio-fertilizer application technology for promotion and application in green rice production.