水稻白叶枯病抗性基因克隆及育种利用
收稿日期: 2025-10-26
网络出版日期: 2026-05-11
基金资助
福建省属公益类科研院所基本科研项目(2020R1023007);福建省属公益类科研院所基本科研项目(2023R11010021-1);福建省科技重大专项(2020R11010016-3);福建省农业科学院科技创新团队(CXTD2021001);福建省农业科学院青年科技创新团队(CXTD2021005-3);福建省5511协同创新工程项目(XTCXGC2021001)
Cloning and Breeding Utilization of Resistance Genes to Bacterial Blight in Rice
Received date: 2025-10-26
Online published: 2026-05-11
傅世元, 杨德青, 杨德卫 . 水稻白叶枯病抗性基因克隆及育种利用[J]. 中国稻米, 2026 , 32(3) : 65 -70 . DOI: 10.3969/j.issn.1006-8082.2026.03.011
Rice bacterial blight, caused by the Gram-negative bacterium Xanthomonas oryzae pv. oryzae(Xoo), is a bacterial disease that affects rice worldwide, significantly reducing both yield and quality. This disease is widespread in China, and its pathogen is characterized by rapid transmission and high mutability. This paper begins by outlining the inheritance patterns of resistance genes against rice bacterial blight. It then details recent advances in the identification of these resistance genes and the application of gene editing technology in breeding for blight resistance. Finally, the molecular mechanisms of resistance are summarized, and future prospects for further mining and functional characterization of resistance genes, as well as their utilization in breeding, are discussed. The aim is to provide a reference for in-depth research on resistance breeding and the genetic mechanisms of rice bacterial blight.
Key words： rice; bacterial blight; resistance gene; breeding utilization
| [1] | BATOOL W, NORVIENYEKU J, YI W, et al. Disruption of non-classically secreted protein (MoMtp) compromised conidiation and pathogenesis of Magnaporthe oryzae[J]. Journal of Integrative Agriculture, 2024, 23(8):2 686-2 702. |
| [2] | 徐坚, 沈颖, 王华弟, 等. 水稻白叶枯病的发生危害与综合防治技术探讨[J]. 中国稻米, 2016, 22(2):65-67. |
| [3] | 徐羡明, 曾列先, 伍尚忠. 广东野生稻种质资源对白叶枯病的抗性鉴定[J]. 广东农业科学, 1986(5):29-31. |
| [4] | 马伦, 杨大兵, 杜雪树, 等. 分子标记辅助选择创制长粒香型抗病水稻光温敏核不育系[J]. 杂交水稻, 2025, 40(2):24-33. |
| [5] | CAO Y L, DUAN L, LI H J, et al. Functional analysis of Xa3/Xa26 family members in rice resistance to Xanthomonas oryzae pv. oryzae[J]. Theoretical and Applied Genetics, 2007, 115(7):887-895. |
| [6] | WANG C L, ZHANG X P, FAN Y L, et al. Xa23 is an executor R protein and confers broad-spectrum disease resistance in rice[J]. Molecular Plant, 2015, 8(2):290-302. |
| [7] | JI C H, JI Z Y, LIU B, et al. Xa1 allelic R genes activate rice blight resistance suppressed by interfering TAL effectors[J]. Plant Communications, 2020, 1(4):100 087. |
| [8] | IYER A S, MCCOUCH S R. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance[J]. Molecular Plant-Microbe Interactions, 2004, 17(12):1 348-1 354. |
| [9] | LIU Q, YUAN M, ZHOU Y, et al. A paralog of the MtN3/saliva family recessively confers race-specific resistance to Xanthomonas oryzae in rice[J]. Plant, Cell & Environment, 2011, 34(11):1 958-1 969. |
| [10] | HU K M, CAO J B, ZHANG J, et al. Improvement of multiple agronomic traits by a disease resistance gene via cell wall reinforcement[J]. Nature Plants, 2017, 3(3):17 009. |
| [11] | 胡珂鸣. 水稻主效抗白叶枯病基因Xa4的克隆和功能分析以及Xa3/Xa26介导的抗白叶枯病信号通路成员的鉴定[D]. 武汉: 华中农业大学, 2015. |
| [12] | 彭小群, 王梦龙. 水稻白叶枯病抗性基因研究进展[J]. 植物生理学报, 2022, 58(3):472-482. |
| [13] | CHEN X, LIU P, MEI L, et al. Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice[J]. Plant Communications, 2021, 2(3):100 143. |
| [14] | 孙振彪, 符辰建, 胡小淳, 等. 优质抗白叶枯病两系杂交水稻晶两优3987的选育与应用[J]. 农业科技通讯, 2022(2):223-225. |
| [15] | 夏贤仁, 姬广海, 张世光. 基因聚合品种对云南水稻白叶枯病的抗性分析[J]. 石河子大学学报(自然科学版), 2004, 22(suppl1):24-26. |
| [16] | 陈玲, 刘丽, 王波, 等. 水稻抗白叶枯病Xa47基因家族的发掘和抗病性鉴定[C]// 第二十届中国作物学会学术年会论文摘要集,2023:113. |
| [17] | OLIVA R, JI C, ATIENZA-GRANDE G, et al. Broad-spectrum resistance to bacterial blight in rice using genome editing[J]. Nature Biotechnology, 2019, 37(11):1 344-1 350. |
| [18] | YOSHIMURA S, YAMANOUCHI U, KATAYOSE Y, et al. Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation[J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(4):1 663-1 668. |
| [19] | HE Q, LI D, ZHU Y, et al. Fine mapping of Xa2, a bacterial blight resistance gene in rice[J]. Molecular Breeding, 2006, 17(1):1-6. |
| [20] | SUN X, CAO Y, YANG Z, et al. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein[J]. The Plant Journal, 2004, 37(4):517-527. |
| [21] | JIANG G H, XIA Z H, ZHOU Y L, et al. Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIAγ1[J]. Molecular Genetics and Genomics, 2006, 275(4):354-366. |
| [22] | 章琦, 施爱农, 王春莲, 等. 水稻白叶枯病(Xanthomonas campestris pv. oryzae)抗性遗传研究Ⅲ.两个成株抗性基因Xa-6与Xa-3的等位性分析[J]. 作物学报, 1991, 17(3):233-237. |
| [23] | TIAN D S, WANG J X, ZENG X. The rice TAL effector-dependent resistance protein Xa10 triggers cell death and calcium depletion in the endoplasmic reticulum[J]. The Plant Cell, 2014, 26(1):497-515. |
| [24] | CHU Z H, FU B Y, YANG H, et al. Targeting xa13, a recessive gene for bacterial blight resistance in rice[J]. Theoretical and Applied Genetics, 2006, 112(3):455-461. |
| [25] | 张标明. 水稻抗白叶枯病主效基因Xa14的克隆与功能分析[D]. 武汉: 华中农业大学, 2020. |
| [26] | SONG W Y, WANG G L, CHEN L L, et al. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21[J]. Science, 1995, 270(5243):1 804-1 806. |
| [27] | WANG S, LIU W, LU D, et al. Distribution of bacterial blight resistance genes in the main cultivars and application of Xa23 in rice breeding[J]. Frontiers in Plant Science, 2020, 11:555 228. |
| [28] | 程奇. 水稻白叶枯病隐性抗病基因xα25的功能验证[D]. 武汉: 华中农业大学, 2016. |
| [29] | GU K Y, YANG B, TIAN D S, et al. R gene expression induced by a type-Ⅲ effector triggers disease resistance in rice[J]. Nature, 2005, 435(7045):1 122-1 125. |
| [30] | KIM S M, REINKE R F, HUN J. Xa40, a novel NLR gene conferring resistance to African strains of Xanthomonas oryzae pv. oryzae[J]. Theoretical and Applied Genetics, 2020, 133(9):2 745-2 756. |
| [31] | LU Y, ZHONG Q, XIAO S, et al. A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice[J]. Frontiers in Plant Science, 2022, 13:1 037 901. |
| [32] | 李舟. 水稻抗白叶枯病基因Xa48(t)分离克隆及种质创制[D]. 昆明: 云南大学, 2022. |
| [33] | 万建民. 中国水稻分子育种现状与展望[J]. 中国农业科技导报, 2007, 9(2):1-9. |
| [34] | 危文亮, 赵应忠. 分子标记在作物育种中的应用[J]. 生物技术通报, 2000, 16(2):12-16. |
| [35] | 朱玉君, 樊叶杨, 王惠梅, 等. 应用分子标记辅助选择培育兼抗稻瘟病和白叶枯病的水稻恢复系[J]. 分子植物育种, 2014, 12(1):17-24. |
| [36] | 潘晓飚, 陈凯, 张强, 等. 分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系[J]. 作物学报, 2013, 39(9):1 582-1 593. |
| [37] | 陈志伟, 官华忠, 王晓方, 等. 三基因聚合改良恢复系福恢673的稻瘟病抗性[J]. 生物工程学报, 2019, 35(5):895-904. |
| [38] | 朱永生, 蔡秋华, 官华忠, 等. 多基因聚合改良杂交稻恢复系福恢676的稻瘟病抗性[J]. 科学通报, 2023, 68(21):2 643-2 652. |
| [39] | 杨德卫, 何旎清, 黄凤凰. 利用分子标记辅助选择聚合水稻抗病基因Pigm-1和Xa23[J]. 西北农林科技大学学报(自然科学版), 2023, 51(11):37-45. |
| [40] | 樊祥瑞, 王俊燕, 梁丽亚, 等. 基于CRISPR/Cas系统的多重基因编辑与调控技术[J]. 生物工程学报, 2023, 39(6):2 449-2 464. |
| [41] | TAO H, SHI X, HE F, et al. Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes[J]. Journal of Integrative Plant Biology, 2021, 63(9):1 639-1 648. |
| [42] | SHA G, SUN P, KONG X, et al. Genome editing of a rice CDP-DAG synthase confers multipathogen resistance[J]. Nature, 2023, 618(7967):1 017-1 023. |
| [43] | XU Y, BAI L, LIU M, et al. Identification of two novel rice S genes through combination of association and transcription analyses with gene-editing technology[J]. Plant Biotechnology Journal, 2023, 21(8):1 628-1 641. |
| [44] | ANDAYA C B, RONALD P C. A catalytically impaired mutant of the rice Xa21 receptor kinase confer spartial resistance to Xanthomonas oryzae pv oryzae[J]. Physiological & Molecular Plant Pathology, 2003, 62(4):203-208. |
| [45] | KOU Y J, WANG S P. Broad-spectrum and durability: understanding of quantitative disease resistance[J]. Current Opinion in Plant Biology, 2010, 13:181-185. |
| [46] | IYER-PASCUZZI A S, JIANG H, HUANG L, et al. Genetic and functional characterization of the rice bacterial blight disease resistance gene xa5[J]. Phytopathology, 2008, 98(3):289-295. |
| [47] | CHEN S, HUANG Z, ZENG L, et al. High-resolution mapping and gene prediction of Xanthomonas oryzae pv. oryzae resistance gene Xa7[J]. Molecular Breeding, 2008, 22(3):433-441. |
| [48] | STREUBEL J, PESCE C, HUTIN M, et al. Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae[J]. New Phytologist, 2013, 200(3):808-819. |
| [49] | LUO D, HUGUET-TAPIA J C, RABORN R T, et al. The Xa7 resistance gene guards the rice susceptibility gene SWEET14 against exploitation by the bacterial blight pathogen[J]. Plant Communications, 2021, 2(3):100 164. |
| [50] | KASHIHARA K, ONOHATA T, YARIUCHI R, et al. The overexpression of OsSRO1a, which encodes an OsNINJA1- and OsMYC2-interacting protein, negatively affects OsMYC2-mediated jasmonate signaling in rice[J]. Plant Cell Reports, 2020, 39(4):489-500. |
| [51] | WU L F, GOH M L, SREEKALA C, et al. Xa27 depends on an amino-terminal signal-anchor-like sequence to localize to the apoplast for resistance to Xanthomonas oryzae pv. oryzae[J]. Plant Physiology, 2008, 148(3):1 497-1 509. |
| [52] | ELLUR R K, KHANNA A, et al. Marker-aided incorporation of Xa38, a novel bacterial blight resistance gene, in PB1121 and comparison of its resistance spectrum with xa13+Xa21[J]. Scientific Reports, 2016, 6:29 188. |
| [53] | HUANG F, HE N, YU M, et al. Identification and fine mapping of a new bacterial blight resistance gene, Xa43(t), in Zhangpu wild rice (Oryza rufipogon)[J]. Plant Biology, 2023, 25(3):433-439. |
| [54] | NELSON R, WIESNER-HANKS T, WISSER R, et al. Navigating complexity to breed disease-resistant crops[J]. Nature Reviews Genetics, 2018, 19(1):21-33. |
| [55] | QIU T, WEI S, FANG K, et al. The atypical D of transcriptional factor OsDes1 contributes to stay-green, grain yield, and disease resistance in rice[J]. Science Advances, 2024, 10(34): eadp0345. |
| [56] | DENG Y W, ZHAI K R, XIE Z, et al. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355(6328):962-965. |
/
| 〈 |
|
〉 |