
水稻苗期恶苗病抗性综合评价及抗病种质筛选
收稿日期: 2025-11-17
网络出版日期: 2026-05-11
基金资助
三亚中国农业科学院国家南繁研究院“南繁专项”(YDLH2501);水稻生物育种全国重点实验室“揭榜挂帅”项目(2025JB102);海南省重点研发项目(ZDYF2023XDNY086);浙江省“尖兵”“领雁” 研发攻关计划项目(2023C04024);“中国农业科学院科技创新工程”资助
Comprehensive Evaluation of Bakanae Disease Resistance at the Seedling Stage and Screening of Resistant Rice Germplasm
Received date: 2025-11-17
Online published: 2026-05-11
水稻恶苗病是一种由藤仓镰孢菌(Fusarium fujikuroi)引起的种传真菌病害,其主要症状是植株徒长,严重时可导致死苗。目前,该病害的防治主要依赖化学农药。而关于水稻种质资源恶苗病抗病性的评价仍存在多样化、无统一标准的问题。。苗期是决定恶苗病发生与否的关键阶段。为此,本研究以99份水稻种质资源(包括早籼稻45份、晚粳稻30份及三系不育系24份)为材料,通过测定苗期的徒长率和死苗率,并转换成恶苗病抗性综合指数,用以系统评价这些种质在苗期的抗病性。结果表明,在99份种质中,仅2份表现为抗病。其中,早籼稻品系中凡早E67的综合指数为1.8,评价为“抗病”;晚粳稻品种浙粳98的综合指数为3.8,评价为“中抗”。其余97份感病材料中,中感、感病和高感种质分别占总数的22.22%、16.16%和59.60%。此外,粳稻材料的整体抗性水平高于籼稻。本研究建立的综合指数能够较全面反映水稻种质在苗期对恶苗病的抗性水平,并成功筛选出中凡早E67和浙粳98这两份抗病种质。本研究结果可为水稻恶苗病的抗性鉴定与抗性育种提供参考。
陈玲, 王文秀, 林文英, 梁丽梅, 欧阳由男, 童涛, 刘国永, 季芝娟 . 水稻苗期恶苗病抗性综合评价及抗病种质筛选[J]. 中国稻米, 2026 , 32(3) : 81 -85 . DOI: 10.3969/j.issn.1006-8082.2026.03.014
Rice bakanae disease, caused by the seed-borne fungus Fusarium fujikuroi, is a fungal disease primarily characterized by symptoms such as excessive elongation of seedlings, which can lead to plant death in severe cases. Currently, chemical control remains the primary method for managing this disease. The evaluation of bakanae disease standards for resistance in rice germplasm resources remains diverse and lacks unifies standards. The seedling stage is a critical period determining the occurrence of bakanae disease. To address this, a comprehensive resistance index was developed based on the elongation rate and seedling death rate at the seedling stage, using 99 rice germplasm resources (including 45 early indica rice, 30 late japonica rice, and 24 three-line sterile lines) to evaluate their resistance. The results revealed that only two out of the 99 germplasms exhibited resistance. Among them, the early indica rice line ‘Zhongfanzao E67’ had a comprehensive index of 1.8, rated as “resistant”, while the late japonica rice variety ‘Zhegeng 98’ had an index of 3.8, rated as “moderately resistant”. Among the remaining 97 susceptible materials, moderately susceptible, susceptible, and highly susceptible germplasms accounted for 22.22%, 16.16%, and 59.60% of the total, respectively. Additionally, japonica rice materials overall demonstrated a higher level of resistance compared to indica rice. The comprehensive index established in this study effectively reflects the resistance level of rice germplasms to bakanae disease at the seedling stage and has successfully identified two resistant germplasECEChese findings provide valuable insights for resistance identification and disease resistance breeding targeting rice bakanae disease.
| [1] | 欧玉苹, 朱峰, 王继春, 等. 粳稻资源与水稻恶苗病菌互作亲和性分析[J]. 吉林农业大学学报, 2024. |
| [2] | WULFF E G, SØRENSEN J L, LÜBECK M, et al. Fusarium spp. associated with rice bakanae: Ecology, genetic diversity, pathogenicity and toxigenicity[J]. Environmental Microbiology, 2010, 12(3):649-657. |
| [3] | 王晓莉, 李哲, 叶文武, 等. 江苏省13个地区水稻种子携带4种不同恶苗病菌的LAMP检测[J]. 南京农业大学学报, 2020, 43(5):846-852. |
| [4] | 邵培珺, 卞康亚, 张玉, 等. 盐城地区氰烯菌酯防治小麦赤霉病、水稻恶苗病效果评价[J]. 江苏农业科学, 2023, 51(22):121-125. |
| [5] | 陈水生. 水稻臻香丝苗恶苗病综合防治技术与效果分析[J]. 粮油与饲料科技, 2024(6):34-36. |
| [6] | FIYAZ R A, KRISHNAN S G, RAJASHEKARA H, et al. Development of high throughput screening protocol and identification of novel sources of resistance against bakanae disease in rice (Oryza sativa L.)[J]. Indian Journal of Genetics and Plant Breeding, 2014, 74(4):414. |
| [7] | SHAKEEL Q, MUBEEN M, SOHAIL M A, et al. An explanation of the mystifying bakanae disease narrative for tomorrow's rice[J]. Frontiers in Microbiology, 2023, 14:1 153 437. |
| [8] | 邵美红, 金水丰, 赵玲玲, 等. 晒种与药剂浸种对水稻种子发芽及恶苗病防控效果的影响[J]. 中国稻米, 2025, 31(2):87-91. |
| [9] | ZHAN L W, CHEN L, HOU Y X, et al. Bakanae disease resistance in rice: Current status and future considerations[J]. Agronomy, 2024, 14(7):1 507. |
| [10] | 邵丽华, 李鹏. 水稻对恶苗病菌侵染响应的蛋白质组分析[J]. 中国农业科技导报(中英文), 2025, 27(6):126-135. |
| [11] | 吕彬. 水稻抗恶苗病的鉴定方法研究[J]. 作物学报, 1996, 22(5):629-632. |
| [12] | 郑镐燮, 吕彬, 吴润植, 等. 水稻恶苗病抗病性筛选方法的初步研究[J]. 植物保护学报, 1993, 20(4):289-293. |
| [13] | 季芝娟, 马良勇, 李西明, 等. 水稻品种恶苗病抗性快速鉴定方法研究[J]. 浙江农业科学, 2008, 49(1):89-91. |
| [14] | 季芝娟. 水稻恶苗病抗性相关基因的鉴定及多抗基因的聚合育种利用[D]. 沈阳: 沈阳农业大学, 2016. |
| [15] | 季芝娟, 曾宇翔, 梁燕, 等. 水稻恶苗病抗性研究进展[J]. 中国水稻科学, 2021, 35(1):1-10. |
| [16] | 中华人民共和国农业部. 水稻品种试验稻瘟病抗性鉴定与评价技术规程:NY/T 2646-2014[S]. 北京: 中国标准出版社, 2014. |
| [17] | 陈玲, 杨长登, 梁燕, 等. 粮食作物真菌类病害及其抗性鉴定方法研究进展[J]. 浙江农业科学, 2024, 65(11):2 650-2 658. |
| [18] | JI H, KIM T H, LEE G S, et al. Mapping of a major quantitative trait locus for bakanae disease resistance in rice by genome resequencing[J]. Molecular Genetics and Genomics, 2018, 293(3):579-586. |
| [19] | 于艳敏, 武洪涛, 刘海英, 等. 水稻品种恶苗病抗性评价与筛选[J]. 农学学报, 2024, 14(7):1-5. |
| [20] | 郑镐燮, 吕彬, 吴润植. 水稻品种资源对恶苗病抗性鉴定研究[J]. 黑龙江农业科学, 1993(3):11-13. |
| [21] | VOLANTE A, TONDELLI A, ARAGONA M, et al. Identification of bakanae disease resistance loci in japonica rice through genome wide association study[J]. Rice, 2017, 10:29. |
| [22] | TADASANAHALLER P S, BASHYAL B M, YADAV J, et al. Identification and characterization of Fusarium fujikuroi pathotypes responsible for an emerging bakanae disease of rice in India[J]. Plants, 2023, 12(6):1 303. |
| [23] | 中国水稻研究所. 一种水稻恶苗病的高效接种方法及其应用:CN202210180562.3[P]. 2024-03-19. |
| [24] | 季芝娟, 马良勇, 李西明, 等. 水稻种质资源恶苗病抗性鉴定[J]. 浙江农业科学, 2008, 49(5):590-592. |
| [25] | 黎定军, 罗宽, 陈寘. 水稻对恶苗病的抗性与病原菌致病性的研究[J]. 植物病理学报, 1993, 23(4):29-33. |
/
| 〈 |
|
〉 |