The genetic diversity in three wild rice species and bamboo, cultivated rice and O. longistaminata were assessed, and compared by ISSR method to determine their phylogenetic relationships, discuss the evolution direction of three kinds of Yunnan wild rice. The results indicated that a total of 464 alleles were detected among all the materials by 33 strips of ISSR primers, 14.1 polymorphic bands were amplified of the average of each primer. Rich genetic diversity existed in the three wild rice species at the aggregate level. The number of polymorphic loci（Np）, the percentage of polymorphic loci（p）, the effective number of alleles（Na）, observed number of alleles（Ne）, Nei 's genetic diversity index （H） and Shannon index （I） were 357, 76.74%, 1.7694±0.4217, 1.3391±0.3637, 0.2037±0.1851 and 0.3180±0.2532. The coefficient of gene differentiation （Gst） was 0.0968 among the three wild rice species while the gene flow （Nm） was 0.4627, which shown that the gene exchanged at low level among three wild rice species and the genetic divergence was relatively high among rice varieties. UPGMA cluster analysis revealed the evolutionary process of three wild rice, O. meyeriana differentiats first, following by the O. Officinalis, O. rufipogon differentiats at the latest.
CHEN Ling, YIN Fu-You, LI Wei-Jiao, LI Ding-Qin, ZHANG Dun-Yu, ZHONG Qiao-Fang, CHEN Yue, KE Xue, HUANG Xing-Qi, CHENG Zai-Quan-*
. Study on Genetic Diversity and Phylogeny Evolution of Three Wild Rice Species in Yunnan[J]. China Rice, 2014
, 20(3)
: 30
-35
.
DOI: 10.3969/j.issn.1006-8082.2014.03.006
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