In order to find the colored-rice cultivars with stronger aroma synthesis potential among the 7 rice cultivars, nitrogen-containing compounds such as L–proline concentration, total nitrogen concentration, soluble protein concentration, soluble sugar concentration of brown rice were compared and analyzed. The results showed that: according to the total nitrogen concentration in decreasing order, the colored-rice cultivars numbered as Shangnonghongjing, Shangnongheinuo, Hongxiangyu, Baijupei, Lvdao and Heijupei, Hongyujupei. According to the soluble sugar concentration in decreasing order, the colored-rice cultivars numbered as Shangnonghongjing, Hongyujupei, Hongxiangyu, Baijupei, Lvdao, Heijupei and Shangnongheinuo. According to the soluble protein concentration in decreasing order, the colored-rice cultivars numbered as Hongyujupei, Heijupei, Baijupei, Shangnongheinuo, Lvdao, Shangnonghongjing and Hongxiangyu. According to the L-proline concentration in decreasing order, the colored-rice cultivars numbered as Baijupei, Lvdao, Hongyujupei, Shangnongheinuo, Hongxiangyu, Heijupei and Shangnonghongjing; and almost all the differences of the cultivars were significant （p＜0.01）. What’s more, L-proline concentration of cultivars named Shangnonghongjing, Shangnongheinuo, Hongyujupei and Lvdao correlated their total nitrogen content positively, while L-proline concentration of cultivars named Shangnongheinuo, Lvdao, Heijupei and Hongyujupei correlated their soluble sugar concentration negatively, especially the cultivars named Heijupei and Lvdao correlated significantly; L-proline concentration of cultivars named Shangnonghongjing, Shangnongheinuo, Lvdao, Hongyujupei correlated their soluble protein concentration negatively and correlated their total nitrogen positively. In a conclusion, the cultivars named Shangnongheinuo, Lvdao, Shangnonghongjing and Hongyujupei have the stronger aroma synthesis potential than others.
YANG Shu-Ying-1, ZOU Ying-Bin-2*, LIU Shao-Kun-3, ZHAO Ze-Sheng-4, LI Zheng-1, PENG Qian-1
. Comparative Study on the Content of the Nitrogen-containing Compounds and Soluble Sugar from the Special Colored-rice Cultivars[J]. China Rice, 2015
, 21(2)
: 8
-12
.
DOI: 10.3969/j.issn.1006-8082.2015.02.003
[1] 陈文，王磊，唐艳芳，等. 黑优粘米酶解水提液延缓衰老作用研究[J]. 上海农学院学报，1996，14（4）：258-261.
[2] 赵则胜，赖来展.中国特种稻[M]. 上海：上海科学技术出版社，1995.
[3] Seok H N，Sun P C，Mi Y K，et a1. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars [J]. Food Chem，2006，9（4），613-620.
[4] 莫识美. 特种稻类型及其开发利用[J]. 广西农学报，2000（3）：30-32.
[5] 胡标林，余守武，万勇，等. 功能性水稻的研究与开发进展[J]. 江西农学报，2005，17（3）：74-78.
[6] 迟明梅，方伟森. 浅谈营养米和功能米[J]. 粮食加工，2005（5）：26-28.
[7] 何光华，袁祚廉，郑家奎. 水稻籽粒蛋白质含量及其产量的遗传效应[J]. 西南农业学报，1995，8：4-9.
[8] 张名位，彭仲明. 特种稻蛋白质含量的遗传效应分析[J]. 中国青年农业科学学术年报，1997：141-146.
[9] 邵启明. 两系杂交籼稻蛋白质含量的研究[J]. 西南农业大学学报，1992，14（1）：39-41.
[10] Hawkins H J, Lewis O. Effect of NaCl salinity, nitrogen form, calcium and potassium content on nitrogen uptake and kinetics in Triticum aestivum L. cv.Gamtoos[J]. New Phytol, 1993, 124：171-177.
[11] Gouia H, Chorbal M H, Touraine B. Effects of NaCl on flow of N and mineral ions and on NO-3 reduction rate within whole plants of saltsensitive bean and salt-tolerant cotton[J]. Plant Physiol, 1994. 105：1409-1418.
[12] Parida A K, Das A B. Effects of NaCl stress on nitrogen and phosphorous metabolism in a true mangrove Bruguiera parviflora grown under hydroponic culture[J]. J Plant Physiol, 2004, 161：921-928.
[13] Dluzniewska P, Gessler A, Dietrich H, et al. Nitrogen uptake and metabolism in Populus x canescens as affected by salinity[J]. New Phytol. 2007, 173：279-293.
[14] Ehlting B, Dluzniewska P, Dietrich, H, et al. Interaction of nitrogen nutrition and salinity in Grey poplar[J]. Plant Cell Environ, 2007, 30：796-811.
[15] Yang S Y, Zou Y B, Liang Y Z, et al. Role of soil total nitrogen in aroma synthesis of traditional regional aromatic rice in China[J]. Field Crops Res, 2012, 125:151-160.
[16] 王德仁，卢婉芳，陈苇，等. 施氮对稻米蛋白质、氨基酸含量的影响[J]. 植物营养与肥料学报，2001，7（3） ：353-356.
[17] 陆引里，周众. 贵州中海拔地区水稻高产中的钾素效应研究[J].西南农业大学学报：自然科学版，2003，25（4）：360-362.
[18] Toshiro H. the relation of the rice taste pepper and the content of mineral element[J] . Jpn J Crop Sci, 1998, 67：405-411.
[19] 李晓鸣. 矿质镁对水稻产量及品质影响的研究[J]. 植物营养与肥料学报，2002，8（1）：125-126.
[20] 龚玉琴，杨金明，白锦红，等. 硅、硫、锌、锰肥配施对水稻品质及产量的影响[J]. 土壤肥料，2004（6）：17-24.
[21] Sriseadka T, Wongpornchai S, Kitsawatpaiboon P. Rapid method for quantitative analysis of the aroma impact compound, 2-Acetyl-1-pyrroline, in fragrant rice using automated headspace gas chromatography[J]. J Agric Food Chem, 2006, 54：8183-8189.
[22] Buttery R G, Juliano B O, Ling L C. Cooked Rice Aroma and 2-Acetyl-1-pyrroline[J]. J Agric Food Chem, 1983, 31：823-826.
[23] Suprasanna P, Ganapathi T R, Ramaswmy N K, et al. Aroma synthesis in cell and callus cultures of rice[J]. Rice Genetics News, 2000, 15：123-125.
[24] Romanczyk L J, McClelland C A, Post L S, et al. Formation of 2-acetyl-1-pyrroline by several Bacillus cereus strains isolated from cocoa fermantation boxes[J]. J Agric Food Chem, 1995, 43：469-475.
[25] Yoshihashi T, Huong N T T, Inatomi H. Precursors of 2-acetyl-1-pyrroline, a potent flavor compound of an aromatic rice variety[J]. J Agric Food Chem, 2002, 50：2001-2004.
[26] Huang T C, Teng C S, Chang J L, et al. Biosynthetic mechanism of 2-Acetyl-1-pyrroline and its relationship with Δ'-pyrroline-5-carboxylic acid and methylglyoxal in aromatic rice（Oryza sativa L.）callus[J]. J Agric Food Chem, 2008, 56：7399-7404
[27] Lam H M, Coschigano K T, Oliveira I C. The moleculargenetics of nitrogen assimilation into amino acids in higher plants. Annual Review Plant Physiological [J]. Plant Mol Biol, 1996, 47：569-93.
[28] Bradbury L, Gillies S, Brushett D.et al. Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice [J]. Plant Mol Biol, 2008, 68：439-449.
[29] Ahmed M U, Frye E B, Degenhardt T P, et al. N-epsilon-（carboxyethyl） lysine, a product of the chemical modification of proteins by methylglyoxal increases with age in human lens proteins [J].Biochem J, 1997, 324：565-570.
[30] Huang T C, Huang Y W, Hung H J, et al.δ1-Pyrroline-5-carboxylic acid formed by proline dehydrogenase from the Bacillus subtilis ssp. natto expressed in Escherichia coli as a precursor for 2-acetyl-1-pyrroline [J]. J Agric Food Chem, 2007, 55：5097-5102.
[31] 顾建明，许晓惠.香米样香成分的研究现状和展望[J]. 上海大学学报：自然科学版，2002，8（3）：227-231.
[32] Barneix A J, Amozis P A, Guitman M R. The regulation of nitrogen accumulation in the grain of wheat plants[J]. Physiol Plant, 1992, 86: 609-615.
