Using the optimum regression design, the effects of seeding date （X1）, seeding rate （X2）, urea rate （X3） and nitrogen proportion in various growing stages （X4） were studied on 8 agronomic traits of japonica late hybrid rice Yongyou 538 by direct seeding. The results showed that the order of the cultivation effects was X1＞X2＞X4＞X3. There were various significant effects of seeding date to 6 agronomic traits （full heading date, seed setting rate, yield, etc）, seeding rate to 7 traits （maximum seedlings, total grains per panicle, yield, etc）, urea rate to 4 traits （tillerings, effective panicles, etc） and nitrogen proportion to 6 traits （plant height, 1000-grain weight, yield, etc）, and had interactions among factors. The analysis by the full factorial simulation screen indicated that in order to obtain higher rice yield ≥ 750 kg/667 m2, the optimal cultivation requirements should be adopted as follows: seeding date June 2-5, seeding rate 1.25~1.41 kg/667 m2, amount of urea 36.3~40.0 kg/667 m2, and nitrogen proportion 28%~32% in seedling stage, 32%~28% in tillering stage, 18%~22% in booting stage, and 22%~18% in flowering stage.