Analysis was conducted on the activity of peroxidase （POD）, catalase （CAT） and superoxide dismutase（SOD ） and Malondialdehyde （MDA） content in the leaves of rice variety Kongyu 131 after low temperature treatments. The results showed that the activity of protective enzymes （SOD, CAT, POD） of the leaves increased with the decrease of temperature, the activity of the three kinds of protective enzyme increased and then decreased with the extension of treatment time. The activity of SOD and CAT showed a decline trend with the increase of recovery time, but POD enzyme activity showed opposite trend. Malondialdehyde （MDA） content increased under the low temperature treatment during booting stage, and increased with the decrease of temperature and increase of treatment time, and decreased with the increase of recovery time. The lower temperature and longer duration treatment made a severe influence on rice leaf. Rice leaf treated with shorter duration in the higher temperature can basically returned to normal growth under the action of protective enzyme.