As seedling raising development in plastic trays, it is becoming more and more difficult to get soil from field because of the destruction of vegetation and soil. Soilless substrate rice seedling raising was a new technique, which replaced the soil by high temperature fermentation substrate and avoided the work of get soil. The seedling quality, growth and yield of Zhonglongjing 1, a Japonica rice variety, in cold region of Northeast China were determined by using the YELIN brand soilless substrate in rice nursery. The study was carried out in 291 Farm, Heilongjiang. The results indicated that the application of YELIN brand soilless substrate can improve the seedling quality, promote the growth of roots, turn green early, reduce the labor intensity, save labor costs, and improve the quality of rice tiller population. Compared with the conventional nutrient soil seedling, the rice yield of soilless substrate raising seedling with machine transplanting was increased by the increase of the effective panicles, total grains and 1000-grain weight.