Evaluation of Rice Blast Resistance in Perennial Rice
1st author: heyifei@mail.ynu.edu.cn
Received date: 2020-11-23
Online published: 2021-01-20
Perennial rice has been developed successful by utilizing the rhizome trait of the wild rice species, Oryza longistaminata and demonstrated widely, exhibiting broad-spectrum resistance to rice blast. To understand the reason of resistance to blast of perennial rice, disease investigation in famer's field, inoculation identification and molecular detection of rice blast resistant genes were carried out among nine accessions including five perennial rice varieties （lines） as Perennial Rice 23（PR23）, Yunda24 （PR24）, Yunda25 （PR25）, Yunda101 （PR101）, Yunda107 （PR107）, male parent O. longistaminata, female parent RD23, F1（RD23/O. longistaminata） and Lijiangxintuanheigu（LTH）. The results showed that all the five perennial rice varieties（lines）, their male parent O. longistaminata and the original F1（RD23/O. longistaminata） had high resistance to rice blast, while their female parent RD23 showed high susceptibility, which indicated that the rice blast resistance gene of these five perennial rice lines may originated from O. longistaminata. The resistance of PR23 and PR25 might owing to the Pi5 gene and Pita-2 locus from O. longistaminata, and the resistance of PR24 might due to the pita-2 locus from O. longistaminata, while that of PR107 might inherited from O. longistaminata with the Pi5 gene and Pish locus. However, none of the known rice blast resistance genes or loci mentioned in this study were harbored by PR101, speculated that the unknown genes from O. longistaminata inherited by PR101 archived its blast resistance. The results of this study provided a basis and strategy for blast disease resistance breeding, perennial rice varieties distribution and plant protection technology decision in future.
Key words： perennial rice; rice blast; resistance evaluation; Oryza longistaminata
Yifei HE, Shiwen QIN, Shilai ZHANG, Guangfu HUANG, Jing ZHANG, Qinzhong YANG, Fengyi HU . Evaluation of Rice Blast Resistance in Perennial Rice[J]. China Rice, 2021 , 27(1) : 9 -13 . DOI: 10.3969/j.issn.1006-8082.2021.01.003
| [1] | 谭秀芳,沈玮囡,任广乾,等. 水稻稻瘟病发生特点及防治措施研究进展[J]. 农业科技通讯,2018(7):237-239. |
| [2] | 曹妮,陈渊,季芝娟,等. 水稻抗稻瘟病分子机制研究进展[J]. 中国水稻科学,2019,33(6):489-498. |
| [3] | 华丽霞,何炼,蒋秋平,等. 稻瘟病抗性基因特异性分子标记的开发及应用进展[J]. 分子植物育种,2016,14(10):2 739-2 748. |
| [4] | TAO D, SRIPICHITT S.Preliminary report on transfer traits of vegetative propagation from wild rice species to oryza sativa via distant hybridization and embryo rescue[J]. The Kasetsart J, Natural Sci, 2000, 34: 1-11. |
| [5] | 胡凤益. 长雄野生稻(Oryza longistaminata)地下茎分子定位和遗传研究[D]. 重庆:西南农业大学,2002. |
| [6] | 胡凤益. 长雄野生稻地下茎基因Rhz2、Rhz3精细定位及地下茎全基因组表达谱分析[D]. 北京:中国农业科学院,2010. |
| [7] | HUANG G, QIN S, ZHANG S, et al.Performance, economics and potential impact of perennial rice PR23 relative to annual rice cultivars at multiple locations in Yunnan province of China[J]. Sustainability, 2018, 10:1086. |
| [8] | XU P, DONG L, ZHOU J, et al.Identification and mapping of a novel blast resistance gene Pi57(t) in Oryza longistaminata[J]. Euphytica, 2015, 205(1): 95-102 . |
| [9] | International Rice Research Institute (IRRI). Standard evaluation system for rice[S]. 2002. |
| [10] | 华丽霞,汪文娟,陈深,等. 抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发[J]. 中国水稻科学,2015,29(3):305-310. |
| [11] | 高利军,高汉亮,颜群,等. 4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J]. 杂交水稻,2010,25(suppl1):294-298. |
| [12] | RAMKUMAR G, SRINIVASARAO K, MOHAN K, et al.Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pikh)[J]. Mol Breed, 2011, 27(1): 129-135. |
| [13] | 刘开强,伍豪,颜群,等. 水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J]. 西南农业学报,2016,29(6):1 241-1 244. |
| [14] | COSTANZO S, JIA Y.Sequence variation at the rice blast resistance gene Pi-km locus: implications for the development of allele specific markers[J]. Plant Sci, 2010, 178(6): 523-530. |
| [15] | KOIDE Y, EBRON L, KATO H, et al.A set of near-isogenic lines for blast resistance genes with an indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background[J]. Field Crop Res, 2011, 123(1): 19-27. |
| [16] | LI W, LEI C, CHENG Z, et al.Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding[J]. Mol Breed, 2008, 22(1): 141-149. |
| [17] | 苏世杰,刘二明,李小娟,等. 稻瘟病菌致病性变异研究进展[J]. 江西植保,2006(3):111-115. |
| [18] | HE R, SALVATO F, PARK J, et al.A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement[J]. BMC Plant Biol, 2014, 14(1): 1-20. |
/
| 〈 |
|
〉 |