Marker-Assisted Selection for Hybrid Rice Restorer Line R153 Conferring Resistance to Blast
1st author: zhuyujun@caas.cn
Received date: 2020-09-24
Online published: 2021-01-20
The rice blast is one of the most serious diseases in rice production. Breeding for disease resistant varieties is the most effective and efficient method to reduce the disease damage. In this study, one rice candidate restorer line, R153, harboring the rice blast resistance gene Pi25 was selected through the approach of molecular marker-assisted selection using segregating population derived from cross between the Hui11-32 and BL108 （the donor of the blast resistance gene Pi25）. Tightly linked DNA marker Si13070C and functional marker CAP3/BglⅡ were used. The blast resistance identification was conducted in a rice blast disease hotspot location, Shanghang County, Fujian Province, China. R153 showed a good resistance to rice blast in the evaluation and provided a new resource for rice breeding.
Key words： rice; rice blast; marker-assisted selection; Pi25; restorer line
Yujun ZHU, Derun HUANG, Yeyang FAN, Jieyun ZHUANG, Bo SHEN . Marker-Assisted Selection for Hybrid Rice Restorer Line R153 Conferring Resistance to Blast[J]. China Rice, 2021 , 27(1) : 95 -97 . DOI: 10.3969/j.issn.1006-8082.2021.01.018
| [1] | 赫中娜,毛雪琴,柴荣耀,等. 国家长江中下游稻区品种区域试验籼稻稻瘟病抗性分析[J]. 中国水稻科学,2019,33(2):152-157. |
| [2] | WANG B H, EBBOLE D J, WANG Z H.The arms race between Magnaporthe oryza and rice: Diversity and interaction of Avr and R genes[J]. Journal of Integrative Agriculture, 2017, 16(12): 2 746-2 760. |
| [3] | JIANG H, LI Z, LIU J, et al.Development and evaluation of improved lines with broad-spectrum resistance to rice blast using nine resistance genes[J]. Rice, 2019, 12: 29. |
| [4] | CHEN Q, ZENG G, HAO M, et al.Improvement of rice blast and brown planthopper resistance of PTGMS line C815S in two-line hybrid rice through marker-assisted selection[J]. Molecular Breeding, 2020, 40: 21. |
| [5] | 陈志伟,官华忠,王晓方,等. 三基因聚合改良恢复系福恢673的稻瘟病抗性[J]. 生物工程学报,2019,35(5):837-846. |
| [6] | MI J, YANG D, CHEN Y, et al.Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice[J]. Rice, 2018, 11: 11. |
| [7] | XIAO W M, PENG X, LUO L X, et al.Development of elite restoring lines by integrating blast resistance and low amylose content using MAS[J]. Journal of Integrative Agriculture, 2018, 17(1): 16-27. |
| [8] | CHEN J, SHI Y, LIU W, et al.A Pid3 allele from rice cultivar Gumei 2 confers resistance to Magnaporthe oryzae[J]. Journal of Genetics and Genomics, 2011, 38: 209-216. |
| [9] | 朱玉君,亓芳丽,应杰政,等. 应用分子标记辅助选择培育抗稻瘟病杂交稻亲本[J]. 福建稻麦科技,2010,28(1):1-3. |
| [10] | ZHENG K, HUANG N, BENNETT J, et al.PCR-based marker-assisted selection in rice breeding[M]. IRRI Discussion Paper Series No.12. Los Banos, Philippines: International Rice Research Institute, 1995. |
| [11] | WANG H M, CHEN J, SHI Y F, et al.Development and validation of CAPS markers for marker-assisted selection of rice blast resistance gene Pi25[J]. Acta Agronomica Sinica, 2012, 38(11): 1 960-1 968. |
| [12] | 陈锦文,谢旺有,王天生,等. 人工注射接种及田间病圃鉴定水稻对稻瘟病的抗病性[J]. 广西植保,2015,28(4):6-10. |
| [13] | 岂长燕,许兴涛,马建,等. 抗稻瘟病基因Pib、Pita、Pi5、Pi25和Pi54在我国水稻微核心种质中的分布[J]. 植物遗传资源学报,2019,20(5):1 240-1 246. |
| [14] | 余守武,郑学强,范天云,等. 分子标记辅助选育具抗稻瘟病基因Pi25的光温敏核不育系[J]. 中国稻米,2013,19(3):15-17. |
| [15] | 涂诗航,周鹏,郑轶,等.分子标记辅助选择Pi25基因选育抗稻瘟病三系不育系[J].分子植物育种,2015,13(9):1 911-1 917. |
| [16] | 董瑞霞,王洪飞,董练飞,等. 分子标记辅助选择改良水稻不育系臻达A及其杂交种的稻瘟病抗性[J]. 植物遗传资源学报,2017,18(3):573-586. |
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