Jianghuai region is one of the main grain producing areas in China,which is rich in temperature, light and water resources. The region is located in the northern and southern climate transition zone, in which the types of diseases, insect pests and weeds are different. Therefore, it is very important to explore the green prevention and control technology of diseases, insect pests and weeds to ensure grain safety production. There are various crop planting patterns in the Jianghuai region, in which the rice-wheat rotation cropping system is the most important one. According to the occurrence regularity and damage characteristics of the main targets pests and weeds in rice-wheat rotation system, the agricultural ecological technology and comprehensive application technology of green inputs, pesticides and machinery were integrated to form the green prevention and control technology for diseases, insect pests and weeds. This technology has been successfully demonstrated and applied in Jianghuai region in recent years. The present study introduced this technology mode in detail in order to provide technical support for sustainable development of regional grain production.