In order to compare the yield and nitrogen use efficiency of Yongyou 1540 under different nitrogen reduction modes, a filed experiment was carried out in two sites representing different soil type in east Zhejiang single-season rice area in 2019. The results showed that compared with the artificial spreading fertilization treatment, the number of grains per panicle was significantly increased by the mechanized side-deep fertilizer treatment, and the average yield of rice increased by 3.0%~8.2%. Under the condition of reducing nitrogen fertilizer by 20% （192 kg/hm²）, the artificial spreading fertilization mode reduced grain yield of rice. However, the mechanized side-deep fertilizer mode could ensure grain yield and significantly increase N use efficiency. Its average yield reached 11.8 t/hm², moreover, the N recovery efficiency （NRE）, N agronomy efficiency （NAE） and N partial factor productivity （NPFP） reached 36.9%, 15.0 kg/kg and 61.7 kg/kg, respectively. Therefore, the indica-japonica hybrid rice Yongyou 1540 should be popularized and applied in the mechanized side-deep fertilizer mode with nitrogen fertilizer reduction in eastern Zhejiang, nitrogen could be used as basal and panicle fertilizer.