Rice blast is one of the three most important rice diseases. Using rice blast resistance genes to breed new varieties is the most economical and effective measure to control the occurrence of rice blast, and is of great significance to the safety of rice production. Based on the identification of the leaf blast and panicle blast resistance levels of 63 main rice varieties in Zhejiang Province, the specific molecular markers of 10 cloned rice blast resistance genes Pi1, Pi5, Pi2, Pi9, Pia, Pizt, Pigm, Pib, Pik and Pikh were used to identify the gene composition of these 63 varieties for resistance to rice blast, and further analyzed the correlation between resistance genes and resistance to rice blast. The resistance identification results showed that among 63 varieties, the leaf blast resistant varieties accounted for 66.7% and the ear blast resistant varieties accounted for 50.8%. Rice blast resistance genes identified showed that 60 of 63 the main cultivated varieties had more than one resistance genes, 45 of which contained Pizt and Pib genes, and 43 varieties contained Pi2 and Pi5 genes. In addition, the distribution frequency of disease-resistant genes Pia, Pik, Pi1, Pi2, Pizt, Pikh, Pi5 and Pib among the main rice varieties in Zhejiang Province is relatively high, while the distribution frequency of Pigm and Pi9 is very low, only 3.2%. This study revealed the composition of the rice blast resistance genes of the main rice varieties in Zhejiang Province and the contribution of the rice blast resistance genes to the improvement of resistance to rice blast, provided an important reference for the breeding of new disease-resistant varieties in Zhejiang Province