In order to explore the effects of the measures of storing seedlings before transplanting on the seedlings quality and yield of rice, a field experiment was carried out by 16 branches of Heilongjiang Beidahuang Agricultural Co., Ltd. in 2020. The results showed that storing seedlings before transplanting was beneficial to slow down seedlings, increase root number, promote early tillering, and increase yield. After transplanting, there are fewer holes in the field, which reduces the amount of labor for seedling replenishment. Among them, the effect of storing seedlings for 4 days before transplanting was the best, and the yield was increased by 7.5 kg/667 m² and the benefit was increased by 48.0 yuan/667 m² compared with the control.