Under the background of agriculture eco-friendly development, the fertilizer application decreased constantly in China, which made the goal of “zero growth of the fertilizer rates by 2020” achieved in advance. However, it needs further study whether the long-term reduction of fertilizers application would have an impact on rice yield. Based on an 8-year experiment in the paddy fields, we conducted the effects of long-term position nitrogen （N） fertilizer reduction on the yield and N utilization of rice among the different amounts of N applied （N0, N160, N210, N260 and N315, representing 0, 160, 210, 260, 310 kg/hm² N respectively）. The results showed that compared with the N260 treatment, the yield of the N160 treatment decreased year by year, and the difference reached a significant level（p<0.05）. The yield of N210 treatment decreased by 0.69%~1.34%, while The yield of N315 treatment increased by 0.23%~0.26% in some years and decreased by 0.05%-1.92% in the other, and the differences did not reach a significant level. The overuse or underuse of N fertilizer would tend to cause the reduction of rice yield. Relation analgsis showed when the N rate was 237.39 kg/hm², the grain N uptake reached peak level. When the N rate was lower than 232.64 kg/hm², the N harvest index remained at 70.31%. When the N rate was 230.73 kg/hm², the N recovery efficiency was the highest at 38.89%. When the N rate was 227.63 kg/hm², the N agronomic efficiency reached peak at 15.56 kg/kg. N partial factor productivity and N use efficiency increased with the reduction of N application rates. Soil N supply accounted for more than 53.36% of the rice N uptake. When the N rate was lower than 225.95 kg/hm², there was a risk of depletion of soil N pool. Considering the yield and the average fertilizer use efficiency over the years, we recommended that the N application rate in the test area would be 225.95-232.64 kg/hm², which could achieve the long-term stable yield.