The effects of different fertilization treatments on the yield and related traits of high altitude japonica rice （2700 m） in Yunnan were studied because of its low yield. The results showed as follows: the yield of treatments of postponed N-fertilizer application, promoted N-fertilizer after former control, replaced by slow controlled release fertilizer and increased panicule fertilizer were 6.50 t/hm², 5.98 t/hm², 5.63 t/hm², 5.57 t/hm², and significantly increased by 24.05%, 14.12%, 7.44% and 6.30% compared with the control, respectively. Postponed N-fertilizer application （base fertilizer 7.5 t/hm² and compound fertilizer 300 kg/hm², without tillering fertilizer, promoting flower fertilizer applying urea 45 kg/hm², protecting flower fertilizer applying urea 45 kg/hm²） promoted the differentiation of secondary stem number and increased population spikelets. At full heading stage, the SPAD difference of inverted 3 leaves and inverted 4 leaves was the smallest,and LAI was the largest, which resulted in the maximum yield increase.