13 hybrid rice varieties selected from high-quality rice sterile line Quan 211S were used to analyze the heat resistance of Quanliangyou series varieties and the effects of high temperature stress on rice milling quality, appearance quality and cooking and eating quality as well as the correlation between heat tolerance and main quality traits in the smart greenhouse for high temperature treatment. The data demonstrated that most of the Quanliangyou series varieties had relatively strong heat tolerance. The heat resistance coefficient was extremely significantly positively correlated with the head rice rate and alkali spreading value, significantly positively correlated with the gel consistency and extremely significantly negatively correlated with chalkiness. After high temperature stress, the head rice rate, alkali spreading value, gel consistency and amylose content of Quanliangyou series varieties were generally decreased, and the chalkiness were increased. The variation of main rice quality indicators such as head rice rate, chalkiness, amylose content, alkali spreading value and gel consistency was less in rice varieties with strong heat resistance than in rice varieties that are relatively sensitive to high temperature under high temperature stress. The study lays a foundation for breeding high temperature-resistant and high-quality rice varieties.