Improving Blast Resistance of Rice CMS Line Taonong 1A By Pi9-based Marker-assisted Selection Strategy
Received date: 2022-08-13
Online published: 2023-01-17
To improve blast resistance of the rice cytoplasmic male sterile（CMS） line Taonong 1A and its maintainer Taonong 1B, a PCR-based co-dominant InDel marker, CoInDF1R2, was developed according to the sequence information of the broad-spectrum and durable blast resistance gene Pi9, then was used in marker-assisted selection backcross breeding practice. The marker CoInDF1R2 amplified a 354 bp DNA band from the genome of 75-1-127， while the PCR product size from both genomes of Taonong 1A and Taonong 1B was about 470 bp. Greenhouse inoculation assay was performed using fifteen Magnaporthe oryzae strains isolated from different rice areas at home and abroad, the Pi9 gene donor rice line 75-1-127 was showed much higher resistance frequency （86.67%） than that of two acceptor parents Taonong 1A and Taonong 1B （6.67%）, the improved Taonong 1A-Pi9 and Taonong 1B-Pi9 was showed the same resistance frequency as 75-1-127 and with high-level seedling blast resistance in field nursery. By means of integrated field assays, an improved CMS line Taonong 1A-Pi9-1 and its maintainer Taonong 1B-Pi9-1 were screened, with high-level and broad-spectrum blast resistance, complete and stable sterility, high stigma exsertion rate, low enclosed spikelet rate, excellent agronomic and yield traits.
Key words： rice; blast resistance; molecular marker-assisted selection; Taonong 1A; Pi9
LI Bowen, LEI Quanqiang, HUANG Jun, ZHANG Ting, ZOU Yuying, CHE Fanhao, DENG Jiqi, LIU Jinling, LIU Xionglun . Improving Blast Resistance of Rice CMS Line Taonong 1A By Pi9-based Marker-assisted Selection Strategy[J]. China Rice, 2023 , 29(1) : 126 -130 . DOI: 10.3969/j.issn.1006-8082.2023.01.022
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