An experiment was designed to study the effects of different planting densities and different nitrogen application rates on the yield of Huazheyou 261 in late season in Jiaojiang District; The results showed that Huazheyou 261 could achieve the highest yields of 559.58 kg/667 m² and ideal panicle grain structure when the transplanting density was 12 000/667 m² and the pure nitrogen application rate was 14 kg/667 m². The panicle grain structure was 190 100/667 m² of effective panicle, 81.49% of seed setting rate, 139.05 grains per panicle, and 22.03 g of 1 000-grain weight under this treatment.