Nangengnuo 2 is a new late-maturing medium japonica glutinous rice variety, derived from the cross of the new rice line C08 and Zhennuo 19 with high quality and disease resistance, which was bred by Institute of Food Crops, Jiangsu Academy of Agricultural Science, using the method of combination conventional breeding with marker assisted selection for blast resistance genes of Pi-ta and Pi-b. It has the characteristics of moderate plant height, lodging resistance, high and stable yield, fine grain quality, comprehensive resistance and good maturity performance. The average yield of Nangengnuo 2 in the regional test in Jiangsu Province was 672.4 kg/667 m², and the rice quality reached the agricultural industry standard level 1. It showed moderate susceptibility to rice blast disease and stripe disease and moderate resistance to bacterial blight. It was approved by Jiangsu Provincial Variety Approval Committee in 2021 and suitable to grow in the middle area and Ningzhenyang area of Jiangsu Province.