Screening of Phosphorus Solubilizing Bacteria from Rice Rhizosphere Soil and Its Mechanism of Phosphorus Solubilizing
#Co-first author
Received date: 2022-09-05
Online published: 2023-05-23
Phosphorus solubilizing bacteria are important agricultural microbial resources, which can improve the absorption and utilization of phosphorus by increasing the content of available phosphorus in the plant rhizosphere. Two strains of bacteria named Bacillus megaterium WSH-0021 and Priestia aryabhattai were obtained after screening and identification. The bacteria of Bacillus megaterium WSH-0021 and Priestia aryabhattai were entered the logarithmic growth period at the 12th and 8th hours of culture, respectively, and both of them significantly reduced the pH value. The Bacillus megaterium WSH-0021 and Priestia aryabhattai were cultured in liquid medium for identification of phosphorus solubilizing capacity of inorganic and organophosphorus bacteria, and the content of available phosphorus in the medium was increased by 391.78% and 1 799.02% respectively compared with control. These two kinds of bacteria were incubated in soil sample 1 (low phosphorus soil) and soil sample 2 (normal phosphorus soil) for 30 days, the soil sample which only applied bacterial culture medium consider as control. The results showed that the bacterial abundance and microbial biomass phosphorus content of soil sample 1 and soil sample 2 were significantly increased, indicating that bacteria survived and played a role in the soil. After applied Bacillus megaterium WSH-0021, the available phosphorus content in soil sample 1 and soil sample 2 both increased by 21.15% and 11.66%, respectively. After applied Priestia aryabhattai, the available phosphorus content in soil sample 1 and soil sample 2 increased by 13.68% and 12.07%. Further research found that the pH value of the two soil samples both decreased significantly after applied the bacteria, and the activity of soil acid phosphatase increased, indicating that the two bacteria release fixed phosphorus in the soil by regulating soil pH value and acid phosphatase activity. The two bacteria screened in this experiment have a well phosphorus solubilization ability and have strong application potential and value in agricultural production.
PAN Lin, MI ChunXia, XU Qingshan, WEI Qianqian, KONG Yali, ZHU Lianfeng, TIAN Wenhao, JIN Qianyu, ZHANG Junhua, ZHU Chunquan . Screening of Phosphorus Solubilizing Bacteria from Rice Rhizosphere Soil and Its Mechanism of Phosphorus Solubilizing[J]. China Rice, 2023 , 29(3) : 51 -55 . DOI: 10.3969/j.issn.1006-8082.2023.03.009
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