Off-type is an important index in rice variety trial. Although off-type has been clearly defined in DUS testing of new varieties, purity detection of varieties, seed reproduction and regional test of varieties, there are still great ambiguities in the actual operation. In this paper, we discriminated the concept of off-types in different rice variety test, aiming to correctly judge off-types, improve the accuracy of rice variety test, and thus avoid the problems of variety authorization, seed quality and variety certification caused by the misjudgment. In addition, it is proposed to accelerate the application of DNA fingerprinting detection technology, in order to ensure the discrimination of off-types in rice more accurate.