The characteristics of the nationally approved two-line early hybrid rice combinations in the area of the middle and lower reaches of the Yangtze River from 2003 to 2022 were analyzed in this paper. 46 two-line early hybrid rice combinations (26 early-medium ripening combinations and 20 late ripening combinations) had been approved by the national crop variety approval committee of China in the area. The average yields of regional test of early-medium ripening combinations and late ripening combinations were 508.91 and 515.89 kg/667 m², respectively. The regional test yield with approved combinations in recent years was higher. The high-quality compliance rate of the combinations was only 10.87%, and the proportions of the combinations which showed moderately resistant to rice blast and bacterial blight were 15.22% and 4.35%, respectively. 29 combinations were included in the national promotion statistics of major crop varieties. Up to the end of 2019, 18 combinations were extended in more than 66 700 hm², 4 combinations were extended in more than 333 400 hm², and 2 combinations were extended in more than 666 700 hm².