Screening and Identification of Endophytic Bacteria from Oryza minuta and Their Plant Growth-promoting Activities
#Co-author
Received date: 2023-02-17
Online published: 2023-07-26
Oryza minuta is a valuable wild rice resource that has good biostress and non-biostress resistance. The collection of endophytic bacterial strain resources from Oryza minuta could be used for micro-ecosystem research and application in agricultural fertilizer production. In this study, a total of 85 endophytic bacterial strains were isolated, including 43 phosphate-solubilizing bacteria, 19 nitrogen-fixing bacteria, 29 siderophore-producing bacteria and 13 indole-3-acetic acid (IAA)-producing bacteria. Strains OMR2-3 and OML3-4 were identified as Enterobacter cloacae and Enterobacter ludwigii based on morphological identification and molecular identification, respectively, showing the highest IAA productivity with yields of 17.22 mg/L and 16.59 mg/L. The greenhouse experiment showed that strains OMR2-3 and OML3-4 significantly promoted perennial rice growth. These results indicated that abundant endophytic bacteria with plant growth-promoting activities live in Oryza minuta. E. cloacae strain OMR2-3 and E. ludwigii strain OML3-4 have great potential to be developed as microbial fertilizers for perennial rice, which will meet the simplified cultivation model of perennial rice.
YANG Lifan, TIAN Qinglin, GONG Yurui, LI Zhenyuan, LI Qingmao, LI Qinyan, HUANG Liyu, HU Fengyi, QIN Shiwen . Screening and Identification of Endophytic Bacteria from Oryza minuta and Their Plant Growth-promoting Activities[J]. China Rice, 2023 , 29(4) : 78 -83 . DOI: 10.3969/j.issn.1006-8082.2023.04.014
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