Shenyou 28 is a new three-line japonica hybrid rice combination developed by combining BT-type male sterile line Shen21A and restorer line Shenhui 26-28. During the seed production process of Shenyou 28, a high level of meeting between parents at flowering period were obtained by regulating the growth and development process of both parents. We studied the effects of different row ratios and row spacing of female parent on the yield of Shenyou 28 seed production under the condition of both male and female parents being mechanically transplanted, and the effects of different plant spacing of female parent on the yield of Shenyou 28 seed production under the condition of all-in-one machine of male parent machine transplanted (line spacing of 25 cm) and female parent machine direct seeding (line spacing of 20 cm). The results showed that under the condition of parents both mechanically transplanted, the seed production yield was higher when the row ratio of parents was 2∶8-10, and the row spacing was 25 cm×14 cm. Under the condition of male parent machine transplanted and female parent machine direct seeding, the seed production yield was higher when the plant spacing of 16 cm. Optimizing the cultivation methods mentioned above can ensure high yield and efficiency in hybrid japonica rice seed production.