A high-quality two-line hybrid rice combination Shuangliangyou 132 was bred by Hunan Hybrid Rice Research Center derived from the cross between Shuang 1S and Shuanghui 32, which was approved by the National Crop Variety Approval Committee in 2020. On September 21, 2022, experts from Chinese Academy of Sciences, Fujian Academy of Agricultural Sciences and other units conducted investigation and production measurement on the seed production demonstration area of Shuangliangyou 132 in Jianning County, Fujian Province, and the yield reached 6 886.05 kg/hm², achieving a large-scale yield breakthrough in hybrid rice seed production. This paper summarized the characteristics of the male and female parent of the combination, and the key points of high-yield seed production techniques of Shuangliangyou 132, which provided guidance for seed production.