Principle and Progress of CRISPR/Cas System in the Detection of Genetically Modified Plants and Their Products
1st author: wangweixia@caas.cn
Received date: 2023-06-17
Online published: 2023-11-21
With the rapid development of genetically modified plants including transgenic and gene-edited plants, the related detection technologies are also facing more challenges, especially for gene editing products without the introduction of exogenous DNA, its detection is more difficult, and the traditional detection technology cannot be satisfied. The accessory nonspecific cleavage activity of different Cas proteins in the CRISPR-Cas-based detection system is widely used in the field of molecular detection. It can be combined with LAMP and RPA isothermal amplification to achieve accurate and rapid on-site detection, even for single gene editing products for nucleotide mutations. However, the application of the detection system in the detection of plant genetically modified products has just started. This paper reviews the principle of the detection system and its application in genetically modified plants and their products.
WANG Weixia, ZHU Tingheng, LAI Fengxiang, WAN Pingjun, WEI Qi, FU Qiang . Principle and Progress of CRISPR/Cas System in the Detection of Genetically Modified Plants and Their Products[J]. China Rice, 2023 , 29(6) : 21 -27 . DOI: 10.3969/j.issn.1006-8082.2023.06.005
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