Effects of GA3 Priming and KNO2 Priming on the Seedling Establishment, Starch Metabolism and Respiratory Metabolism of Rice
1st author: tan1622840900@163.com
Received date: 2023-10-17
Online published: 2024-05-20
Seed priming, as a treatment technique before sowing, has been widely reported to promote seed germination and seedling growth. Both gibberellin (GA3) and potassium nitrite (KNO2) are involved in the regulation of seed germination. However, the effects of GA3 priming and KNO2 priming on germination and emergence of rice seeds and related physiological and metabolic processes and their combined effects are still unclear. In this study, the conventional rice Xiangzaoxian 45 and hybrid rice Zhuliangyou 4024 were used as test varieties. The effects of water priming (HP), gibberellin priming (GA3), potassium nitrite priming (KNO2), compound priming (GA3+KNO2) and non-priming control (CK) on seed germination, seedling growth, starch degradation metabolism and respiratory metabolism of rice were investigated under growth chamber conditions. The results showed that, compared with CK, priming treatments significantly improved the germination index, vitality index and seedling quality, and the promotion effects of GA3 priming and KNO2 priming were basically better than that of HP priming. At the same time, compared with CK and HP priming, GA3 priming and KNO2 priming significantly increased α-amylase activity and soluble sugar content during seed germination, and increased respiratory oxygen consumption rate, ATP content and glycolytic rate-limiting enzyme activity to a certain extent. There was a certain synergistic effect between the two priming treatments. However, the effect on anaerobic respiration metabolic enzyme activity was not obvious. The above results indicated that GA3 priming and KNO2 priming treatment significantly promoted the growth of rice at the germination stage, and the reason for the improvement may be closely related to the improvement of starch degradation ability and respiratory metabolic activity. However, further studies are needed to reveal the physiological， biochemical and molecular mechanisms of GA3 priming and KNO2 priming affecting starch degradation and respiratory metabolism in the future.
Key words： rice; seed priming; gibberellin; potassium nitrite; germination stage
TAN Biao, REN Muyao, YANG Zhengpeng, XU Jiayi, ZHENG Huabin, TANG Qiyuan, WANG Weiqin . Effects of GA3 Priming and KNO2 Priming on the Seedling Establishment, Starch Metabolism and Respiratory Metabolism of Rice[J]. China Rice, 2024 , 30(3) : 40 -47 . DOI: 10.3969/j.issn.1006-8082.2024.03.006
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