Varieties & Technology

Study on the Improvement of Blast Resistance in Rice Dalixiang by Introducing the Broad-spectrum Resistance Gene Pi9

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  • Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
First author contact:

1st author: 18558168852@163.com;

Received date: 2023-12-13

  Online published: 2024-07-16

Abstract

Dalixiang has won the “China Rice Expo Gold Award” for its excellent rice quality and rich aroma for five consecutive times. However, its high susceptibility to rice blast severely limits its large-scale application in production. Using the male parent of Kongyu 131 containing the broad-spectrum resistance gene Pi9, through conventional hybridization, continuous backcrossing, and molecular marker-assisted selection, we introduced Pi9 gene into Dalixiang. An improved strain was created, which had important traits such as leaf morphology, thousand grain weight, rice quality, and aroma that were basically consistent with Dalixiang. The identification results of the resistance of the improved strain of Dalixiang showed that its blast resistance was significantly improved compared to Dalixiang, meeting the resistance standards for variety production and application, achieving directional improvement of blast resistance in Dalixiang.

Cite this article

ZHANG Xichun, LI Zujun, TANG Huihui, WANG Qian, WU Zhaoxin, WU Jianqiang, ZHU Susong . Study on the Improvement of Blast Resistance in Rice Dalixiang by Introducing the Broad-spectrum Resistance Gene Pi9[J]. China Rice, 2024 , 30(4) : 86 -88 . DOI: 10.3969/j.issn.1006-8082.2024.04.016

References

[1] 国家水稻数据中心. 稻瘟病主效抗性基因列表[DB/OL]. (2015-04-11)[2023-12-13].
[2] LIU G, LU G, ZENG L, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6[J]. Molecular Genetics and Genomics, 2002, 267(4): 472-480.
[3] QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J]. Genetics, 2006, 172(3):1901-1 914.
[4] JIANG N, LI Z Q, WU J, et al. Molecular mapping of the Pi2/9 allelic gene Piz-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson[J]. Rice, 2012, 5(1): 1-7.
[5] 李博文, 雷权强, 黄俊, 等. 分子标记辅助选择Pi9基因改良水稻不育系桃农1A稻瘟病抗性[J]. 中国稻米, 2023, 29(1):126-130.
[6] 张雪梅. 四川省水稻主栽品种抗瘟性评价和内恢99-14抗瘟性遗传分析[D]. 成都: 四川农业大学, 2011.
[7] 邹拓, 耿雷跃, 张薇, 等. 粳稻种质资源稻瘟病抗性及抗性基因分析[J]. 中国稻米, 2022, 28(2):45-50.
[8] ZHENG K L, HUANG N, BENNETT J, et al. PCR-based marker-assisted selection in rice breeding[M]. IRRI Discussion Paper Series No.12. Manila: IRRI, 1995: 1-24.
[9] 陈升. 应用分子标记辅助选择培育广谱、高抗白叶枯病的杂交水稻恢复系[D]. 武汉: 华中农业大学, 2000.
[10] 王亚琦, 孙子淇, 郑峥, 等. 作物分子标记辅助选择育种的现状与展望[J]. 江苏农业科学, 2018, 46(5):6-12.
[11] SERVIN B, MARTIN O C, MEZARD M, et al. Toward a theory of marker-assisted gene pyramiding[J]. Genetics, 2004, 168(1): 513-523.
[12] 倪大虎, 易成新, 李莉, 等. 分子标记辅助培育水稻抗白叶枯病和稻瘟病三基因聚合系[J]. 作物学报, 2008, 34(1):100-105.
[13] 向小娇, 张建, 郑天清, 等. 应用分子标记技术改良京作1号的稻瘟病抗性[J]. 植物遗传资源学报, 2016, 17(4):773-780.
[14] 赖怡帆, 孙君玥, 张旭辉, 等. 分子标记辅助选择Pigm基因改良湘晚籼13号的稻瘟病抗性[J]. 湖南农业大学学报(自然科学版), 2019, 45(2):113-117.
[15] 蒋志谦. 优质水稻新品系大粒香的选育及应用[J]. 贵州农业科学, 2008, 36(5):12-13.
[16] 江健, 刘霞, 詹金碧, 等. 施用钾肥对优质稻大粒香产量和稻瘟病抗性的影响[J]. 农技服务, 2012, 29(8):924.
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