The utilization of heterosis between indica and japonica rice subspecies is an effective way to improve rice yield. Huazhongyou 9326 is a new indica-japonica hybrid rice variety bred by Zhejiang Academy of Agricultural Sciences and Zhejiang Wuwangnong Seed Shareholding Co., Ltd. It was approved by National Crop Variety Approval Committee in 2022. In order to comprehensively understand the production characteristics of this variety and accelerate its rational promotion and application, we analyzed the yield, quality and resistance of Huazhongyou 9326 through methods such as coefficient of variation, Shukla variance analysis, high stability coefficient, regression analysis, and fitness with molecular markers of resistance alleles, based on the data of “the regional test of single-season late japonica varieties in the middle and lower reaches of the Yangtze River” in 2020 and 2021，and compared the prolificacy, stability and adaptability to the control variety Jiayou 5. In the regional and production trials, the average yield of Huazhongyou 9326 increased by 13.45% and 10.95% respectively compared to Jiayou 5, with the proportion of increased production points being 96.15% and 100.0%, respectively. The three-years high stability coefficient of Huazhongyou 9326 was 91.78%, which was increased 6.49% than that of Jiayou 5, indicating that Huazhongyou 9326 had good stability. According to the quality data, Huazhongyou 9326 is classified as Grade 3 with a steaming and cooking taste value of 85.12. In conclusion, Huazhongyou 9326 is an ideal indica/japonica hybrid rice variety with prolificacy, stability and adaptability.