Mapping of Rice Blast Resistance Gene Based on BSA-seq
Received date: 2023-12-13
Online published: 2024-11-19
In order to explore the genetic loci of blast resistance-related traits in rice, the recombinant inbred lines of rice 2013ZJP-3×Jingning 11 hybridization were used as experimental materials. The resistance to leaf blast and neck blast was identified by artificial inoculation and natural induction during 2022—2023, and QTL was analyzed by BSA-seq method. Phenotypic identification showed that the resistance of F11 population to rice blast was distributed continuously, and most materials showed moderate resistance, preferring to the resistant parent jingning 11. 19 resistant lines and 17 sensitive lines were screened to construct the resistant pool. After sequencing, mutation detection and association analysis, a total of 2 regions to blast resistance were obtained, mainly distributed on chromosome 5 and 7, with a total length of 1.45 Mb. 94 different genes, including 12 frame shift mutations, 82 non-synonymous mutations, 9 frame shift and non-synonymous mutations, were screened out. LOC_Os05g10630, LOC_Os05g10650 and LOC_Os07g08960 genes were predicted to have the greatest correlation with blast resistance in rice.
Key words： rice; blast resistance; BSA-seq; gene mapping
CHEN Li, SUN Jianchang, WANG Xin . Mapping of Rice Blast Resistance Gene Based on BSA-seq[J]. China Rice, 2024 , 30(6) : 35 -41 . DOI: 10.3969/j.issn.1006-8082.2024.06.006
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