Analysis of Variation Types of BADH2 Aroma Genes and Detection of Rice Blast Resistance Genes in 80 Aromatic Rice Materials
1st author: 116523094@qq.com
Received date: 2024-07-31
Online published: 2025-01-13
Using six aroma gene variation types and five rice blast broad-spectrum resistance gene functional markers, we detected the Badh2 aroma gene variant types and rice blast resistance genes in 80 aromatic rice materials. At the same time, we also counted the rice blast identification results of the approved varieties in rice regional trial, and identified the aroma and rice blast phenotype. The results showed that all 80 aromatic rice materials possessed aroma, with 7 samples (accounting for 8.75%) having a 803 bp deletion mutation in exons 4 to 5, and 4 samples (accounting for 5.00%) containing two types of variants with a 3 bp deletion in the 5’UTR region and an 8 bp deletion and 3 bp mutation in exon 7. The remaining 69 samples (accounting for 86.25%) had the variant type of 8 bp deletion and 3 bp mutation in exon 7. Among the 80 aromatic rice materials, the numbers containing the Pita, Pi2, Pi9, Pigm, and Pi1 resistance genes were 57 (71.25%), 12 (15.00%), 4 (5.00%), 1 (1.25%), and 1 (1.25%), respectively. There are 9 materials containing both Pi2 and Pita (accounting for 11.25%), 1 material containing both Pigm and Pita (accounting for 1.25%), and 1 material containing both Pi1 and Pita (accounting for 1.25%). The proportion of materials carrying two resistance genes simultaneously exhibiting disease resistance is higher than that of materials carrying only one resistance gene and those without resistance genes. The research results provide a basis for the application of aromatic rice materials and the pyramiding of rice blast resistance genes, as well as for molecular marker-assisted breeding of aromatic rice.
Key words： aromatic rice; aroma gene; rice blast resistance gene
LI Hu, WU Zishuai, LIU Guanglin, CHEN Chuanhua, LUO Qunchang, ZHU Qinan . Analysis of Variation Types of BADH2 Aroma Genes and Detection of Rice Blast Resistance Genes in 80 Aromatic Rice Materials[J]. China Rice, 2025 , 31(1) : 35 -43 . DOI: 10.3969/j.issn.1006-8082.2025.01.005
| [1] | 严如玉, 甘国渝, 赵希梅, 等. 我国水稻优势产区生产格局及施肥现状研究[J]. 中国稻米, 2023, 29(3):1-8. |
| [2] | 牛兆国. 我国优质水稻生产现状及发展对策[J]. 现代农业科技, 2010(23):343-347. |
| [3] | 张倩倩, 殷春渊, 刘贺梅, 等. 48份水稻骨干材料香味及Badh2变异类型的鉴定[J]. 种子, 2024, 43(1):107-113. |
| [4] | 梁雪雨, 郭敏, 欧旭华, 等. 南方稻区主栽香稻品种香味基因Badh2基因型分析[J]. 广东农业科学, 2023, 50(12):104-111. |
| [5] | 刘广林, 李虎, 吴子帅, 等. 9个广西常规香稻品种香味及香味基因变异类型分析[J]. 中国稻米, 2022, 28(6):54-58. |
| [6] | LORIEUX M, PETROV M, HUANG N, et al. Aroma in rice: Genetic analysis of a quantitative trait[J]. Theoretical and Applied Genetics, 1996, 93(7): 1 145-1 151. |
| [7] | 彭波, 孙艳芳, 陈报阳, 等. 水稻香味基因及其在育种中的应用研究进展[J]. 植物学报, 2017, 52(6):797-807. |
| [8] | BRADBURY L M T, FITZGERALD T L, HENRY R J, et al. The gene for fragrance in rice[J]. Plant Biotechnology Journal, 2005, 3 (3): 363-370. |
| [9] | SHI W W, YANG Y, CHEN S H, et al. Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties[J]. Molecular Breeding, 2008, 22 (2) : 185-192. |
| [10] | SHAO G N, TANG A, TANG S Q, et al. A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice[J]. Plant Breeding, 2011, 130 (2) : 172-176. |
| [11] | HE Q, PARK Y J. Discovery of a novel fragrant allele and develop-ment of functional markers for fragrance in rice[J]. Molecular Breeding, 2015, 35 (11) : 1-10. |
| [12] | MYINT K M, ARIKIT S, WANCHANA S, et al. A PCR-based mark-er for a locus conferring the aroma in Myanmar rice (Oryza sativa L. )[J]. Theoretical and Applied Genetics, 2012, 125 (5) : 887-896. |
| [13] | OOTSUKA K, TAKAHASHI I, TANAKA K, et al. Genetic polymor-phisms in Japanese fragrant landraces and novel fragrant allele do-mesticated in northern Japan[J]. Breed Science, 2014, 64 (2) : 115-124. |
| [14] | 王军, 杨杰, 陈志德, 等. 水稻香米基因标记的开发与应用[J]. 分子植物育种, 2008, 6(6):1 209-1 212. |
| [15] | 闫影, 诸光明, 张丽霞, 等. 水稻香味基因分子标记的开发及应用[J]. 西北植物学报, 2015, 35(2):269-274. |
| [16] | 杨婕, 杨长登, 曾宇翔, 等. 水稻稻瘟病抗性基因挖掘与利用研究进展[J/OL]. 中国水稻科学. |
| [17] | 高清, 张亚玲, 葛欣, 等. 水稻抗稻瘟病基因研究进展[J]. 分子植物育种, 2022, 20(11):3 634-3 643. |
| [18] | 李进斌, 李鼎, 孙一丁, 等. 利用与抗稻瘟病基因Pi1连锁的MRG4766标记鉴定173份云南地方稻种[J]. 分子植物育种, 2012, 10(1):73-79. |
| [19] | 卿冬进, 刘开强, 杨燕宇, 等. 基于PARMS技术的抗稻瘟病基因Pigm分子标记的开发[J]. 西南农业学报, 2018, 31(8):1 617-1 621. |
| [20] | 于苗苗, 戴正元, 潘存红, 等. 广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应[J]. 作物学报, 2013, 39(11):1 927-1 934. |
| [21] | 江南, 王素华, 李智强, 等. 水稻 Pi2/9位点3个抗瘟基因的抗菌谱及稻瘟病菌遗传多样性分析[J]. 湖南农业大学学报, 2012, 38(5):506-510. |
| [22] | YU S B, ALI J, ZHOU S C, et al. From green super rice to green agriculture: Reaping the promise of functional genomics research[J]. Molecular Plant, 2022, 15(1): 9-26. |
| [23] | 宋泽, 余显权, 黎小冰, 等. 应用 MAS 技术改良水稻特异种质大粒溪香的稻瘟病抗性[J]. 种子, 2022, 41(6):18-22. |
| [24] | 曾晓强, 胡慧, 高若愚, 等. 利用分子标记辅助选择改良‘长农粳1号’的稻瘟病抗性[J]. 分子植物育种, 2022, 20(13):4 400-4 406. |
| [25] | 陈萍萍, 游月华, 江巍, 等. 利用分子辅助选择聚合抗稻瘟病基因选育黑糯稻新品系[J]. 农业科技通讯, 2021(10):140-144. |
| [26] | 孙富, 唐梅, 何聪, 等. 分子标记辅助聚合基因Pi1和Pi2改良红色特种籼稻稻瘟病抗性研究[J]. 湖北农业科学, 2018, 57(18):23-27. |
| [27] | 陈志伟, 官华忠, 王宗华, 等. 利用基因聚合技术改良一个优质水稻不育系的稻瘟病抗性[J]. 福建农林大学学报(自然科学版), 2012, 41(5):449-454. |
| [28] | 刘开强, 伍豪, 颜群, 等. 水稻抗稻瘟病基因Pi的特异性分子标记开发及利用[J]. 西南农业学报, 2016, 29(6):1 241-1 244. |
| [29] | 高利军, 高汉亮, 颜群, 等. 4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[C]// 第一届中国杂交水稻大会论文集. 2010:294-298. |
| [30] | DENG Y W, ZHAI K R, XIE Z, et al. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355: 962-965. |
| [31] | MURRAY M G, THOMPSON W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research, 1980, 8 (19):4 321-4 326. |
| [32] | 曾宇, 夏秀忠, 农保选, 等. 广西特色香稻地方品种香味及其香味基因型的鉴定[J]. 南方农业学报, 2017, 48(9): 1548-1 553. |
| [33] | 邹拓, 耿雷跃, 张薇, 等. 水稻抗病虫基因挖掘及聚合育种研究进展[J]. 河北农业科学, 2018, 22(5):47-67. |
| [34] | 赵国超, 谢水锋, 宗士鹏, 等. 利用分子标记辅助选育优质香软水稻新品系“上师大 22 号”及试种[J]. 上海师范大学学报(自然科学版), 2022, 51(2):237-242. |
| [35] | 韦敏益, 黄大辉, 张月雄, 等. 分子标记辅助选育香型优质稻新品种‘桂野香占’[J]. 分子植物育种, 2024, 22(13):4 275-4 284. |
| [36] | 李虎, 刘广林, 吴子帅, 等. 利用分子标记辅助选育优质香稻新品种‘桂香 99’[J]. 分子植物育种, 2023, 21(2):510-516. |
| [37] | 吕军, 蒋洪波, 刘博, 等. 利用分子标记辅助选择聚合水稻Pi-ta和 fgr基因[J]. 分子植物育种, 2022, 20(5):1 581-1 587. |
| [38] | 胡明珠, 杨瑰丽, 刘洪, 等. 香型水稻种质资源筛选与Badh2等位变异分析[J]. 分子植物育种. |
| [39] | KOVACH M J, CALINGACION M N, FITZGERALD M A, et al. The origin and evolution of fragrance in rice (Oryza sativa L. )[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106 (34): 4 444-4 449. |
| [40] | 朴日花, 金永梅, 李萍, 等. 我国北方香型粳稻资源遗传多样性及其香味基因Badh2的等位基因分析[J]. 吉林农业大学学报, 2021, 43(5):507-515. |
| [41] | 陈睿, 陈子强, 凌波, 等. 华南籼稻骨干亲本稻瘟病基因检测与抗性评价[J]. 植物遗传资源学报, 2024, 25(3):386-398. |
| [42] | 张佩胜, 赵春德, 余宁, 等. 稻瘟病抗性基因的克隆及应用研究进展[J]. 中国稻米, 2014, 20(5 ):1-7. |
| [43] | 伍尚忠, 朱小源, 刘斌, 等. 籼稻品种三黄占2号的稻瘟病持久抗性评价与遗传分析[J]. 中国农业科学, 2004, 37(4 ):528-534. |
| [44] | 闫影, 王凯, 周锋利, 等. 长三角地区粳稻种质的稻瘟病抗性基因鉴定及其抗性评价[J]. 核农学报, 2022, 36(1):14-23. |
/
| 〈 |
|
〉 |