A comparative experiment of Yongyou series indica-japonica hybrid rice as ratoon rice was carried out in Ningbo in 2023, aiming to screen new high-yield and high-quality ratoon rice varieties suitable for promotion and cultivation in Eastern Zhejiang, and improve the comprehensive production capacity of ratoon rice in the Eastern Zhejiang and promote the development of the rice industry. Comprehensively considering of the performance of the five tested varieties in terms of two-season yield, regrowth ability, and quality of ratoon rice, Yongyou 4901 and Yongyou 6760 performed particularly well, with moderate maturity, high yield, strong regrowth ability, and excellent quality of ratoon rice, making them suitable for promotion as ratoon rice in the Eastern Zhejiang. Yongyou 1540 also showed good yield and regrowth ability, but its growth period is relatively long, in order to ensure full heading safely in the southern region of Ningbo, scientific cultivation management measures such as early sowing and increasing the height of stubble retention should be taken.